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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2006-12-5
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pubmed:abstractText |
Peripheral benzodiazepine receptors (PBRs) are upregulated on activated microglia. We recently developed a promising positron emission tomography (PET) ligand, [11C]PBR28, with high affinity and excellent ratio of specific to nonspecific binding. We assessed the ability of [11C]PBR28 PET to localize PBRs in a rat permanent middle cerebral artery occlusion (MCAO) model of neuroinflammation. [11C]PBR28 was intravenously administered to rats at 4 and 7 days after permanent MCAO. In all experiments, arterial blood was sampled for compartmental modeling of regional distribution volumes, and rat brains were sampled after imaging for in vitro [3H]PK 11195 autoradiography and histological evaluation. [11C]PBR28 PET and [3H]PK 11195 autoradiography showed similar areas of increased PBRs, especially in the peri-ischemic core. Results from these in vivo and in vitro methods were strongly correlated. In this first study to demonstrate neuroinflammation in vivo with small animal PET, [11C]PBR28 had adequate sensitivity to localize and quantify the associated increase in PBRs.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0304-3940
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pubmed:author |
pubmed-author:BriardEmmanuelleE,
pubmed-author:ChuangDe-MawDM,
pubmed-author:FujitaMasahiroM,
pubmed-author:HongJinsooJ,
pubmed-author:ImaizumiMasaoM,
pubmed-author:InnisRobert BRB,
pubmed-author:KimHyun-JuHJ,
pubmed-author:MusachioJohn LJL,
pubmed-author:PikeVictor WVW,
pubmed-author:RuetzlerChristlC,
pubmed-author:ZoghbiSami SSS
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
411
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
200-5
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pubmed:meshHeading |
pubmed-meshheading:17127001-Acetamides,
pubmed-meshheading:17127001-Animals,
pubmed-meshheading:17127001-Autoradiography,
pubmed-meshheading:17127001-Brain Ischemia,
pubmed-meshheading:17127001-Carbon Isotopes,
pubmed-meshheading:17127001-Image Processing, Computer-Assisted,
pubmed-meshheading:17127001-Isoquinolines,
pubmed-meshheading:17127001-Male,
pubmed-meshheading:17127001-Phenyl Ethers,
pubmed-meshheading:17127001-Positron-Emission Tomography,
pubmed-meshheading:17127001-Rats,
pubmed-meshheading:17127001-Rats, Sprague-Dawley,
pubmed-meshheading:17127001-Receptors, GABA-A,
pubmed-meshheading:17127001-Time Factors,
pubmed-meshheading:17127001-Tissue Distribution
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pubmed:year |
2007
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pubmed:articleTitle |
PET imaging with [11C]PBR28 can localize and quantify upregulated peripheral benzodiazepine receptors associated with cerebral ischemia in rat.
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pubmed:affiliation |
Molecular Imaging Branch, National Institute of Mental Health, National Institutes of Health, Building 1, Room B3-10, 1 Center Drive, MSC 0135, Bethesda, MD 20892-0135, USA. imaizumim@intra.nimh.nih.gov
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Intramural
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