Linked Life Data

17101127

Source:http://linkedlifedata.com/resource/pubmed/id/17101127

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
2006-11-27
pubmed:abstractText
ATP-sensitive potassium (K(ATP)) channels play an important role in the regulation of cerebral vascular tone. In vitro studies using synthetic K(ATP) channel openers suggest that the pharmacological profiles differ between rat basilar arteries and rat middle cerebral arteries. To address this issue, we studied the possible involvement of endothelial K(ATP) channels by pressurized arteriography after luminal administration of synthetic K(ATP) channel openers to rat basilar and middle cerebral arteries. Furthermore, we examined the mRNA and protein expression profile of K(ATP) channels to rat basilar and middle cerebral arteries using quantitative real-time PCR (Polymerase Chain Reaction) and Western blotting, respectively. In the perfusion system, we found no significant responses after luminal application of three K(ATP) channel openers to rat basilar and middle cerebral arteries. In contrast, abluminal application caused a concentration-dependent dilatation of both arteries, that was more potent in basilar than in middle cerebral arteries. Quantitative real-time PCR detected the presence of mRNA transcripts of the K(ATP) channel subunits Kir6.1, Kir6.2, SUR1 and SUR2B, while SUR2A mRNA was barely detected in both rat basilar and middle cerebral arteries. Of the five mRNAs, the expression levels of Kir6.1 and SUR2B transcripts were predominant in both rat basilar and middle cerebral arteries. Western blotting detected the presence of Kir6.1, Kir6.2, SUR1 and SUR2B proteins in both arteries. Densitometric measurements of the Western blot signals further showed higher expression levels of Kir6.1 and SUR2B proteins in rat middle cerebral arteries than was found in rat basilar arteries. In conclusion, our in vitro pharmacological studies showed no evidence for functional endothelial K(ATP) channels in either artery. Furthermore, the results indicate that Kir6.1/SUR2B is the major K(ATP) channel complex in rat basilar and middle cerebral arteries.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0014-2999
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
553
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
254-62
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:17101127-Angiography, pubmed-meshheading:17101127-Animals, pubmed-meshheading:17101127-Basilar Artery, pubmed-meshheading:17101127-Blotting, Western, pubmed-meshheading:17101127-DNA, Complementary, pubmed-meshheading:17101127-DNA Primers, pubmed-meshheading:17101127-Densitometry, pubmed-meshheading:17101127-KATP Channels, pubmed-meshheading:17101127-Male, pubmed-meshheading:17101127-Microscopy, Fluorescence, pubmed-meshheading:17101127-Middle Cerebral Artery, pubmed-meshheading:17101127-Organ Specificity, pubmed-meshheading:17101127-Potassium Channels, Inwardly Rectifying, pubmed-meshheading:17101127-RNA, Messenger, pubmed-meshheading:17101127-Rats, pubmed-meshheading:17101127-Rats, Sprague-Dawley, pubmed-meshheading:17101127-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2006
pubmed:articleTitle
Pharmacological and molecular comparison of K(ATP) channels in rat basilar and middle cerebral arteries.
pubmed:affiliation
Department of Neurology, Glostrup Hospital, University of Copenhagen, DK-2600 Glostrup, Denmark. KEBEPL01@glostruphosp.kbhamt.dk
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't