Source:http://linkedlifedata.com/resource/pubmed/id/17098236
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2006-12-20
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pubmed:databankReference | |
pubmed:abstractText |
The cDNA encoding prepro-thyrotropin-releasing hormone (ppTRH) in a teleost, medaka (Oryzias latipes) was isolated and characterized. The medaka ppTRH cDNA codes for 270 amino acid residues including eight TRH progenitor sequences (-Lys/Arg-Arg-Gln-His-Pro-Gly-Lys/Arg-Arg-). In silico analyses of the medaka genome database predicted that the structure of the medaka ppTRH gene is similar to the ppTRH genes of the other vertebrate species studied to date; consisting of three exons and two introns. Identity of the medaka ppTRH with the other vertebrates is rather low except the sockeye salmon. A molecular phylogenic tree showed that the ppTRH sequences reflected the predicted pattern of species classification. RT-PCR analysis demonstrated ppTRH gene expression in the brain and retina. These results gave some insight into the molecular evolution of ppTRH and physiological functions of TRH in vertebrates.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Thyrotropin-Releasing Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/prepro-thyrotropin-releasing hormone
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0016-6480
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
150
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
364-70
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pubmed:meshHeading |
pubmed-meshheading:17098236-Amino Acid Sequence,
pubmed-meshheading:17098236-Animals,
pubmed-meshheading:17098236-Base Sequence,
pubmed-meshheading:17098236-Brain,
pubmed-meshheading:17098236-Cloning, Molecular,
pubmed-meshheading:17098236-DNA, Complementary,
pubmed-meshheading:17098236-Molecular Sequence Data,
pubmed-meshheading:17098236-Oryzias,
pubmed-meshheading:17098236-Phylogeny,
pubmed-meshheading:17098236-Protein Precursors,
pubmed-meshheading:17098236-Retina,
pubmed-meshheading:17098236-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17098236-Sequence Alignment,
pubmed-meshheading:17098236-Thyrotropin-Releasing Hormone
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pubmed:year |
2007
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pubmed:articleTitle |
Molecular cloning of prepro-thyrotropin-releasing hormone cDNA from medaka (Oryzias latipes).
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pubmed:affiliation |
Department of Biotechnology, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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