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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1991-6-12
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pubmed:abstractText |
Specific binding of antigens to the surface immunoglobulin M (sIgM) triggers B cells with several biochemical events involved in receptor-mediated signal transmission for proliferation and differentiation into antibody-producing cells. Recent studies with the Digitonin lysis method identified the sIgM-associated component, IgM-alpha (B34)/Ig-beta, as the possible candidate for the transducer molecule(s) in the immunoglobulin receptor-mediated signal transmission. The 34 kd protein (B34 or IgM-alpha) of this component is suggested to be encoded by the B cell-specific mb-1 gene. We prepared monoclonal antibodies which recognize the mb-1 gene product (MB-1) and studied the functional role of MB-1 in the signal transmission in B lineage cells. Using murine pre-B lymphoma cells (18-81 and 70Z/3), we demonstrated the early phase increase of the intracellular [Ca2+]i concentration and the subsequent inhibition of the proliferation by the monoclonal anti-MB-1 antibody (11-18-5). These results clearly demonstrate signal transmission through the surface MB-1 molecule on B lineage lymphomas. This MB-1-mediated signal transmission in pre-B cell lines would suggest an alternative function of MB-1 acting at the pre-B cell stage.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD79,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cd79a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, B-Cell
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0953-8178
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
117-26
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1709046-Animals,
pubmed-meshheading:1709046-Antibodies, Monoclonal,
pubmed-meshheading:1709046-Antigens, CD,
pubmed-meshheading:1709046-Antigens, CD79,
pubmed-meshheading:1709046-B-Lymphocytes,
pubmed-meshheading:1709046-Calcium,
pubmed-meshheading:1709046-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1709046-Epitopes,
pubmed-meshheading:1709046-Flow Cytometry,
pubmed-meshheading:1709046-Immunoglobulin M,
pubmed-meshheading:1709046-Lymphocyte Activation,
pubmed-meshheading:1709046-Lymphoid Tissue,
pubmed-meshheading:1709046-Membrane Glycoproteins,
pubmed-meshheading:1709046-Mice,
pubmed-meshheading:1709046-Mice, Inbred BALB C,
pubmed-meshheading:1709046-Rabbits,
pubmed-meshheading:1709046-Rats,
pubmed-meshheading:1709046-Rats, Inbred Lew,
pubmed-meshheading:1709046-Receptors, Antigen, B-Cell,
pubmed-meshheading:1709046-Signal Transduction,
pubmed-meshheading:1709046-Tumor Cells, Cultured
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pubmed:year |
1991
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pubmed:articleTitle |
Signal transmission through the B cell-specific MB-1 molecule at the pre-B cell stage.
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pubmed:affiliation |
Department of Immunology, Saga Medical School, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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