Source:http://linkedlifedata.com/resource/pubmed/id/17087060
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2006-11-7
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pubmed:abstractText |
A ts+ revertant of cold-adapted (ca) strain A/Leningrad/134/47/57--the attenuation donor for live influenza reassortant vaccines--was obtained by passages of the ca strain in chick embryos at nonpermissive temperatures. The ts+ revertant acquired the ability to grow in chick embryos at 40 degrees C and lost the capacity to reproduce there at 25 degrees C. A complementation-recombination test using the fowl plague virus (FPV0 ts-mutants showed the loss of the ts-phenotype in the RNA-segments of ts+ revertants' genome coding for PB2, NP, and NS (NS2) proteins. However, PCR-restriction analysis revealed a true reversion in RNA-segment coding for PB2 protein only. All the investigated mutations in the ts+ revertant genome were preserved. This phenomenon could be explained by the appearance of intragenic and extragenic suppression mutations in the ts+ revertant genome. The data of the complementation-recombination test suggest that reversion of ts-phenotype occurs more frequently due to extra- or intragenic suppression rather than as a result of a true mutation loss. Estimation of the genetic stability of vaccine ca strains of influenza virus should be based on the combined use of PCR-restriction and complementation tests.
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pubmed:language |
rus
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0507-4088
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
51
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
17-22
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:17087060-Adaptation, Physiological,
pubmed-meshheading:17087060-Animals,
pubmed-meshheading:17087060-Chick Embryo,
pubmed-meshheading:17087060-Genetic Complementation Test,
pubmed-meshheading:17087060-Hot Temperature,
pubmed-meshheading:17087060-Influenza A Virus, H2N2 Subtype,
pubmed-meshheading:17087060-Polymerase Chain Reaction,
pubmed-meshheading:17087060-Reassortant Viruses,
pubmed-meshheading:17087060-Recombination, Genetic,
pubmed-meshheading:17087060-Serial Passage,
pubmed-meshheading:17087060-Suppression, Genetic,
pubmed-meshheading:17087060-Viral Proteins,
pubmed-meshheading:17087060-Virus Replication
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pubmed:articleTitle |
[Molecular mechanisms of reversions to the ts+ -phenotype of cold-adapted influenza virus A strains--attenuation donors for live influenza reassortant vaccines].
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pubmed:publicationType |
Journal Article,
English Abstract,
Research Support, Non-U.S. Gov't
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