|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
9
|
|
pubmed:dateCreated |
1975-12-29
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|
pubmed:abstractText |
Penicillinacylase activity was determined in E. coli by the product of benzylpenicillin destruction, i.e. phenylacetic acid formed under the effect of the enzyme. The determination was performed on a chromatograph. The immobile phase consisted of 10 per cent of ethylenglycol edipate on chromosorb A, modified with 2 per cent H3PO4. Nitrogen was used as the gaseous carrier. The method is rapid and handy for mass testing of the cultures with a purpose of detecting penicillinacylase-producing strains. It provided reliable determination of penicillinacylase in the cultures producing simultaneously beta-lactamase, another penicillin-destroying enzyme.
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pubmed:language |
rus
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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|
pubmed:month |
Sep
|
|
pubmed:issn |
0003-5637
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|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
810-3
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|
pubmed:dateRevised |
2006-11-15
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|
pubmed:meshHeading |
|
|
pubmed:year |
1975
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|
pubmed:articleTitle |
[Determination of the penicillin acylase activity of E. coli cultures using a method of gas-liquid chromatography].
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|
pubmed:publicationType |
Journal Article,
Comparative Study,
English Abstract
|
