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pubmed-article:17084642pubmed:abstractTextA novel glucose oxidase (GOX), a flavoenzyme, from Penicillium sp. was isolated, purified and partially characterised. Maximum activities of 1.08U mg(-1)dry weight intracellular and 6.9U ml(-1) extracellular GOX were obtained. Isoelectric focussing revealed two isoenzymes present in both intra- and extracellular fractions, having pI's of 4.30 and 4.67. GOX from Penicillium sp. was shown to be dimeric with a molecular weight of 148kDa, consisting of two equal subunits with molecular weight of 70k Da. The enzyme displayed a temperature optimum between 25 and 30 degrees C, and an optimum pH range of 6-8 for the oxidation of beta-d-glucose. The enzyme was stable at 25 degrees C for a minimum of 10h, with a half-life of approximately 30 min at 37 degrees C without any prior stabilisation. The lyophilized enzyme was stable at -20 degrees C for a minimum of 6 months. GOX from Penicillium sp. Tt42 displayed the following kinetic characteristics: Vmax, 240.5U mg(-1); Km, 18.4mM; kcat, 741 s(-1) and kcat/Km, 40 s(-1)mM(-1). Stability at room temperature, good shelf-life without stabilisation and the neutral range for the pH optimum of this GOX contribute to its usefulness in current GOX-based biosensor applications.lld:pubmed
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pubmed-article:17084642pubmed:authorpubmed-author:SimpsonCClld:pubmed
pubmed-article:17084642pubmed:authorpubmed-author:GardinerN SNSlld:pubmed
pubmed-article:17084642pubmed:authorpubmed-author:WhiteleyCClld:pubmed
pubmed-article:17084642pubmed:authorpubmed-author:JordaanJJlld:pubmed
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pubmed-article:17084642pubmed:volume51lld:pubmed
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pubmed-article:17084642pubmed:year2007lld:pubmed
pubmed-article:17084642pubmed:articleTitleIsolation, purification and characterization of a novel glucose oxidase from Penicillium sp. CBS 120262 optimally active at neutral pH.lld:pubmed
pubmed-article:17084642pubmed:affiliationCSIR Biosciences, Modderfontein, Johannesburg 1645, South Africa. csimpson2@csir.co.zalld:pubmed
pubmed-article:17084642pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:17084642pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed