Source:http://linkedlifedata.com/resource/pubmed/id/17084642
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
2006-12-4
|
pubmed:abstractText |
A novel glucose oxidase (GOX), a flavoenzyme, from Penicillium sp. was isolated, purified and partially characterised. Maximum activities of 1.08U mg(-1)dry weight intracellular and 6.9U ml(-1) extracellular GOX were obtained. Isoelectric focussing revealed two isoenzymes present in both intra- and extracellular fractions, having pI's of 4.30 and 4.67. GOX from Penicillium sp. was shown to be dimeric with a molecular weight of 148kDa, consisting of two equal subunits with molecular weight of 70k Da. The enzyme displayed a temperature optimum between 25 and 30 degrees C, and an optimum pH range of 6-8 for the oxidation of beta-d-glucose. The enzyme was stable at 25 degrees C for a minimum of 10h, with a half-life of approximately 30 min at 37 degrees C without any prior stabilisation. The lyophilized enzyme was stable at -20 degrees C for a minimum of 6 months. GOX from Penicillium sp. Tt42 displayed the following kinetic characteristics: Vmax, 240.5U mg(-1); Km, 18.4mM; kcat, 741 s(-1) and kcat/Km, 40 s(-1)mM(-1). Stability at room temperature, good shelf-life without stabilisation and the neutral range for the pH optimum of this GOX contribute to its usefulness in current GOX-based biosensor applications.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
1046-5928
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
51
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
260-6
|
pubmed:meshHeading |
pubmed-meshheading:17084642-Enzyme Stability,
pubmed-meshheading:17084642-Glucose Oxidase,
pubmed-meshheading:17084642-Hydrogen-Ion Concentration,
pubmed-meshheading:17084642-Isoelectric Focusing,
pubmed-meshheading:17084642-Kinetics,
pubmed-meshheading:17084642-Molecular Weight,
pubmed-meshheading:17084642-Penicillium,
pubmed-meshheading:17084642-Temperature
|
pubmed:year |
2007
|
pubmed:articleTitle |
Isolation, purification and characterization of a novel glucose oxidase from Penicillium sp. CBS 120262 optimally active at neutral pH.
|
pubmed:affiliation |
CSIR Biosciences, Modderfontein, Johannesburg 1645, South Africa. csimpson2@csir.co.za
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|