Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1991-4-26
pubmed:abstractText
An enzyme immunoassay (EIA) was developed to measure serum antibody responses of healthy adult volunteers vaccinated with 40 or 80 micrograms of human immunodeficiency virus type 1 (HIV-1) recombinant gp160 (rgp160) vaccine at 0, 1, 6, and 18 months. This assay, which used purified rgp160 as antigen, was compared with the Biotech/Du Pont HIV-1 Western blot and the Abbott HIV-1 EIA. Of 33 volunteers who received three doses of rgp160 vaccine, seroresponses were detected in 91% by rgp160 EIA, 97% by Western blot, and 30% by HIV-1 EIA. The level of IgG rgp160 EIA antibody (mainly IgG1) peaked after the third immunization; 64% of 33 vaccinees still had detectable antibody by 12 months. The fourth immunization induced anamnestic IgG EIA antibody in 23 of 24 vaccinees, with titers ranging from 1:200 to 1:25,600. Neutralizing antibody was not detected in postvaccination sera by microtiter syncytium formation inhibition assay. Additional testing of sera by EIA indicated that the immune response to the vaccine was directed toward epitopes on both gp120 and gp41. Seroresponses to the immunodominant epitopes on gp41 were infrequent and none were detected to the neutralization epitope in the V3 region of gp120. This highly sensitive EIA is useful for characterizing HIV-1-specific antibody responses induced by an HIV-1 gp160 subunit vaccine.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0889-2229
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1251-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1706607-Adult, pubmed-meshheading:1706607-Amino Acid Sequence, pubmed-meshheading:1706607-Blotting, Western, pubmed-meshheading:1706607-Drug Evaluation, pubmed-meshheading:1706607-Epitopes, pubmed-meshheading:1706607-Gene Products, env, pubmed-meshheading:1706607-HIV Antibodies, pubmed-meshheading:1706607-HIV Antigens, pubmed-meshheading:1706607-HIV Envelope Protein gp120, pubmed-meshheading:1706607-HIV Envelope Protein gp160, pubmed-meshheading:1706607-HIV Envelope Protein gp41, pubmed-meshheading:1706607-HIV-1, pubmed-meshheading:1706607-Humans, pubmed-meshheading:1706607-Immunoenzyme Techniques, pubmed-meshheading:1706607-Immunoglobulin G, pubmed-meshheading:1706607-Kinetics, pubmed-meshheading:1706607-Middle Aged, pubmed-meshheading:1706607-Molecular Sequence Data, pubmed-meshheading:1706607-Neutralization Tests, pubmed-meshheading:1706607-Protein Precursors, pubmed-meshheading:1706607-Vaccines, Synthetic, pubmed-meshheading:1706607-Viral Vaccines
pubmed:year
1990
pubmed:articleTitle
Characterization of serum antibody responses to recombinant HIV-1 gp160 vaccine by enzyme immunoassay. NIAID AIDS Vaccine Clinical Trials Network.
pubmed:affiliation
Department of Pediatrics, School of Medicine, Johns Hopkins University, Baltimore, MD 21205.
pubmed:publicationType
Journal Article, Clinical Trial, Research Support, U.S. Gov't, P.H.S., Controlled Clinical Trial