|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0185117,
umls-concept:C0205263,
umls-concept:C0596508,
umls-concept:C0599851,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C1999216,
umls-concept:C2245722,
umls-concept:C2745888,
umls-concept:C2911684
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2007-1-17
|
|
pubmed:abstractText |
Cultured mouse D3 embryonic stem (ES) cells differentiating into embryoid bodies (EBs) expressed several Wnt isoforms, nearly all isotypes of the Wnt receptor Frizzled and the Wnt/Dickkopf (Dkk) co-receptor low-density lipoprotein receptor-related protein (LRP) type 5. A 4-day treatment with retinoic acid (RA), which promoted neural differentiation of EBs, substantially increased the expression of the Wnt antagonist Dkk-1, and induced the synthesis of the Wnt/Dkk-1 co-receptor LRP6. Recombinant Dkk-1 applied to EBs behaved like RA in inducing the expression of the neural markers nestin and distal-less homeobox gene (Dlx-2). Recombinant Dkk-1 was able to inhibit the Wnt pathway, as shown by a reduction in nuclear beta-catenin levels. Remarkably, the antisense- or small interfering RNA-induced knockdown of Dkk-1 largely reduced the expression of Dlx-2, and the neuronal marker beta-III tubulin in EBs exposed to RA. These data suggest that induction of Dkk-1 and the ensuing inhibition of the canonical Wnt pathway is required for neural differentiation of ES cells.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Dkk1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0022-3042
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
100
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
242-50
|
|
pubmed:dateRevised |
2007-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:17064353-Animals,
pubmed-meshheading:17064353-Blotting, Western,
pubmed-meshheading:17064353-Cell Differentiation,
pubmed-meshheading:17064353-Cells, Cultured,
pubmed-meshheading:17064353-Dose-Response Relationship, Drug,
pubmed-meshheading:17064353-Drug Interactions,
pubmed-meshheading:17064353-Embryo, Mammalian,
pubmed-meshheading:17064353-Gene Expression Regulation, Developmental,
pubmed-meshheading:17064353-Immunohistochemistry,
pubmed-meshheading:17064353-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:17064353-Mice,
pubmed-meshheading:17064353-Neurons,
pubmed-meshheading:17064353-Oligodeoxyribonucleotides, Antisense,
pubmed-meshheading:17064353-RNA, Messenger,
pubmed-meshheading:17064353-RNA, Small Interfering,
pubmed-meshheading:17064353-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17064353-Signal Transduction,
pubmed-meshheading:17064353-Stem Cells,
pubmed-meshheading:17064353-Transfection,
pubmed-meshheading:17064353-Tretinoin,
pubmed-meshheading:17064353-Wnt Proteins
|
|
pubmed:year |
2007
|
|
pubmed:articleTitle |
Expression of the Wnt inhibitor Dickkopf-1 is required for the induction of neural markers in mouse embryonic stem cells differentiating in response to retinoic acid.
|
|
pubmed:affiliation |
Department of Human Physiology and Pharmacology, University of Rome La Sapienza, Rome, Italy.
|
|
pubmed:publicationType |
Journal Article
|
