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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2006-10-25
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pubmed:abstractText |
Fever is an evolutionarily conserved mechanism to improve survival during infection. Previous studies have shown that feverlike temperatures directly enhance the function of murine bone marrow-derived dendritic cells (DCs). In the present study, we examined the response of human monocyte-derived DC to 39.5 degrees C hyperthermia. When primed with toll-like receptor agonists or bacterial extract but not proinflammatory cytokines, hyperthermia specifically enhanced secretion of interleukin (IL)-12p70 by DC, without altering the secretion of IL-10, tumor necrosis factor alpha or IL-1beta. These DC induced significantly higher levels of T-cell proliferation and interferon gamma production in assays of antigen presentation and MLR. Endogenous heat-sock protein 70 colocalized with CD40 in DC exposed to hyperthermic conditions. Recombinant CD40-Fc fusion protein blocked the increase in IL-12p70 secretion by DC primed with bacterial extract and hyperthermia. Thus, DC primed with toll-like receptor-agonists respond to hyperthermia with increased IL-12p70 secretion, mediated by heat-shock protein binding and activation of CD40. The data have important applications for clinical immunotherapy and the mechanism of fever.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/CD40Ig protein, recombinant,
http://linkedlifedata.com/resource/pubmed/chemical/CD83 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HSP70 Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptidoglycan,
http://linkedlifedata.com/resource/pubmed/chemical/Poly I-C,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ribomunyl,
http://linkedlifedata.com/resource/pubmed/chemical/Tetanus Toxoid,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptors
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pubmed:status |
MEDLINE
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pubmed:issn |
1524-9557
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
606-15
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:17063123-Antigen Presentation,
pubmed-meshheading:17063123-Antigens, Bacterial,
pubmed-meshheading:17063123-Antigens, CD,
pubmed-meshheading:17063123-Antigens, CD40,
pubmed-meshheading:17063123-Antigens, CD86,
pubmed-meshheading:17063123-Cell Differentiation,
pubmed-meshheading:17063123-Cell Proliferation,
pubmed-meshheading:17063123-Cytokines,
pubmed-meshheading:17063123-Dendritic Cells,
pubmed-meshheading:17063123-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:17063123-HLA-DR Antigens,
pubmed-meshheading:17063123-HSP70 Heat-Shock Proteins,
pubmed-meshheading:17063123-Hot Temperature,
pubmed-meshheading:17063123-Humans,
pubmed-meshheading:17063123-Immunoglobulins,
pubmed-meshheading:17063123-Interferon-gamma,
pubmed-meshheading:17063123-Interleukin-12,
pubmed-meshheading:17063123-Interleukin-4,
pubmed-meshheading:17063123-Lipopolysaccharides,
pubmed-meshheading:17063123-Lymphocyte Activation,
pubmed-meshheading:17063123-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:17063123-Membrane Glycoproteins,
pubmed-meshheading:17063123-Monocytes,
pubmed-meshheading:17063123-Peptidoglycan,
pubmed-meshheading:17063123-Poly I-C,
pubmed-meshheading:17063123-Recombinant Fusion Proteins,
pubmed-meshheading:17063123-T-Lymphocytes,
pubmed-meshheading:17063123-Tetanus Toxoid,
pubmed-meshheading:17063123-Toll-Like Receptors
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pubmed:articleTitle |
Monocyte-derived DC primed with TLR agonists secrete IL-12p70 in a CD40-dependent manner under hyperthermic conditions.
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pubmed:affiliation |
Centre for Immunology and Cancer Research, Princess Alexandra Hospital, Division of Chemical Engineering, University of Queensland, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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