Source:http://linkedlifedata.com/resource/pubmed/id/17059560
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2007-1-17
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| pubmed:abstractText |
Synaptic scaffolding molecule (S-SCAM) is a synaptic protein, which harbors five or six PSD-95/Discs large/ZO-1 (PDZ), a guanylate kinase and two WW domains. It interacts with NMDA receptor subunits, neuroligin and beta-catenin, and is involved in the accumulation of neuroligin at excitatory synapses. In this study, we have demonstrated S-SCAM is localized at inhibitory synapses in rat primary cultured hippocampal neurons. We have identified beta-dystroglycan (beta-DG) as a binding partner for S-SCAM at inhibitory synapses. WW domains of S-SCAM bind to three sequences of beta-DG. We have also revealed that S-SCAM can interact with neuroligin 2, which is known to be exclusively localized at inhibitory synapses. The WW domains and the second PDZ domain of S-SCAM are involved in the interaction with neuroligin 2. Beta-DG, neuroligin 2 and S-SCAM form a tripartite complex in vitro. Neuroligin 2 is detected in the immunoprecipitates by anti-beta-DG antibody from rat brain. S-SCAM, beta-DG and neuroligin 2 are partially co-localized in rat hippocampal neurons. These data suggest that S-SCAM is associated with beta-DG and neuroligin 2 at inhibitory synapses, and functions as a linker between the dystrophin glycoprotein complex and the neurexin-neuroligin complex.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Guanylate Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Magi2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neural Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/SAPAP1 protein, rat
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0022-3042
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
100
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
154-66
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:17059560-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:17059560-Animals,
pubmed-meshheading:17059560-Blotting, Western,
pubmed-meshheading:17059560-Carrier Proteins,
pubmed-meshheading:17059560-Cells, Cultured,
pubmed-meshheading:17059560-Cercopithecus aethiops,
pubmed-meshheading:17059560-Cricetinae,
pubmed-meshheading:17059560-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:17059560-Embryo, Mammalian,
pubmed-meshheading:17059560-Guanylate Kinase,
pubmed-meshheading:17059560-Hippocampus,
pubmed-meshheading:17059560-Immunohistochemistry,
pubmed-meshheading:17059560-Nerve Tissue Proteins,
pubmed-meshheading:17059560-Neural Cell Adhesion Molecules,
pubmed-meshheading:17059560-Neural Inhibition,
pubmed-meshheading:17059560-Neurons,
pubmed-meshheading:17059560-Protein Structure, Tertiary,
pubmed-meshheading:17059560-Rats,
pubmed-meshheading:17059560-Synapses,
pubmed-meshheading:17059560-Two-Hybrid System Techniques
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| pubmed:year |
2007
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| pubmed:articleTitle |
Synaptic scaffolding molecule (S-SCAM) membrane-associated guanylate kinase with inverted organization (MAGI)-2 is associated with cell adhesion molecules at inhibitory synapses in rat hippocampal neurons.
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| pubmed:affiliation |
Department of Medical Biochemistry, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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