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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0007589,
umls-concept:C0012888,
umls-concept:C0017262,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0185117,
umls-concept:C0208973,
umls-concept:C0441655,
umls-concept:C0870134,
umls-concept:C1511938,
umls-concept:C1516960,
umls-concept:C1517892,
umls-concept:C1527148,
umls-concept:C1704666,
umls-concept:C1998793,
umls-concept:C2911684
|
| pubmed:issue |
6
|
| pubmed:dateCreated |
1991-4-17
|
| pubmed:abstractText |
These studies aimed to determine the expression and functional role of c-myb in erythroid progenitors with different cycling activities. In the first series of experiments the erythroid burst-forming unit (BFU-E) and colony-forming unit (CFU-E) populations from adult peripheral blood (PB), bone marrow (BM), and embryonic-fetal liver (FL) were treated with either c-myb antisense oligomers or 3H-thymidine (3H-TdR). A direct correlation was always observed between the inhibitory effect of anti-myb oligomers and the level of cycling activity. Thus, the inhibitory effect of antisense c-myb on the number of BFU-E colonies was 28.3% +/- 15.8% in PB, 53.4% +/- 9.3% in BM, and 68.2% +/- 24.5% in FL. Both adult and embryonic CFU-E were markedly inhibited (73.2% +/- 10.4% and 74.2% +/- 12.7%). Using highly purified PB progenitors, we observed a similar pattern, although with slightly lower inhibitory effects. In the 3H-TdR suicide assay the killing index of BFU-E was 8.9% +/- 4.2% in PB, 29.4% +/- 6.5% in BM, and 40.1% +/- 9.6% in FL. The values for adult and embryonic CFU-E were 55.7% +/- 7.9% and 60.98% +/- 6.6%, respectively. We then investigated the kinetics of c-myb mRNA level during the erythroid differentiation of highly purified adult PB and FL BFU-E, as evaluated in liquid-phase culture by reverse transcription-polymerase chain reaction. Adult erythroid precursors showed a gradual increase of c-myb mRNA from day 4 through day 8 of culture and a sharp decrease at later times, whereas the expression of c-myb mRNA and protein in differentiation embryonic precursors peaked 2 days earlier. In both cases, c-myb mRNA level peaked at the CFU-E stage of differentiation. Finally, highly purified adult PB BFU-E were stimulated into cycling by a 3-day treatment with interleukin-3 in liquid phase: both the sensitivity to c-myb antisense oligomers and the 3H-TdR suicide index showed a gradual, strictly parallel increase. Under the same experimental conditions a progressive increase of the mRNA level of DNA polymerase alpha was observed. These observations suggest that in early erythroid differentiation c-myb activation is associated with the progression of progenitors into the S phase of the cell cycle, as well as to the synthesis of DNA polymerase alpha.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antisense Elements (Genetics),
http://linkedlifedata.com/resource/pubmed/chemical/DNA Polymerase II,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-3,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myb,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myc,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Directed DNA Polymerase,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium
|
| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
|
| pubmed:issn |
0006-4971
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
15
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| pubmed:volume |
77
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| pubmed:geneSymbol |
c-myb,
c-myc
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1181-90
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:1705831-Antisense Elements (Genetics),
pubmed-meshheading:1705831-Base Sequence,
pubmed-meshheading:1705831-Cell Differentiation,
pubmed-meshheading:1705831-DNA Polymerase II,
pubmed-meshheading:1705831-Erythroid Precursor Cells,
pubmed-meshheading:1705831-Gene Expression,
pubmed-meshheading:1705831-Hematopoietic Stem Cells,
pubmed-meshheading:1705831-Humans,
pubmed-meshheading:1705831-Interleukin-3,
pubmed-meshheading:1705831-Molecular Sequence Data,
pubmed-meshheading:1705831-Oligonucleotides, Antisense,
pubmed-meshheading:1705831-Polymerase Chain Reaction,
pubmed-meshheading:1705831-Proto-Oncogene Proteins,
pubmed-meshheading:1705831-Proto-Oncogene Proteins c-myb,
pubmed-meshheading:1705831-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:1705831-RNA, Messenger,
pubmed-meshheading:1705831-RNA-Directed DNA Polymerase,
pubmed-meshheading:1705831-Thymidine,
pubmed-meshheading:1705831-Tritium
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| pubmed:year |
1991
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| pubmed:articleTitle |
Antisense myb inhibition of purified erythroid progenitors in development and differentiation is linked to cycling activity and expression of DNA polymerase alpha.
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| pubmed:affiliation |
Department of Hematology-Oncology, Istituto Superiore di Sanità, Rome, Italy.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|