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rdf:type |
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lifeskim:mentions |
umls-concept:C0003250,
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0033684,
umls-concept:C0185117,
umls-concept:C0205419,
umls-concept:C0242643,
umls-concept:C0276037,
umls-concept:C0680730,
umls-concept:C1148554,
umls-concept:C1519421,
umls-concept:C1704222,
umls-concept:C1704939,
umls-concept:C1880022,
umls-concept:C2911684
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pubmed:issue |
4
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pubmed:dateCreated |
1991-3-22
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pubmed:abstractText |
We isolated an IgG2a murine monoclonal antibody (MAb) termed MAb57, specifically reactive with multi-drug-resistant (MDR) human cells. Its specificity toward the MDRI gene product (P-glycoprotein) has been demonstrated by the concordant segregation of the MAb57 epitope with the MDRI gene in interspecific mouse x human cell hybrids, and the reactivity of several different MDRI gene-expressing cells with MAb57, particularly insect cells acutely infected with a baculovirus encoding the MDRI gene. MAb57 can be used to detect, by flow cytometry, variations in the relative drug-resistance levels of several MDR KB and CEM cell variants. This immunological probe has also proven useful in selectively destroying MDR target cells in an antibody-dependent cell-mediated (ADCC) assay system as well as in detecting P-glycoprotein expression in normal and malignant tissues and cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0020-7136
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pubmed:author |
pubmed-author:BarbuDD,
pubmed-author:CenciarelliCC,
pubmed-author:CianfrigliaMM,
pubmed-author:CurrierS JSJ,
pubmed-author:DupuisM LML,
pubmed-author:GermannU AUA,
pubmed-author:GottesmanM MMM,
pubmed-author:MarianiMM,
pubmed-author:MorroneSS,
pubmed-author:RamoniCC,
pubmed-author:RutherfordA VAV,
pubmed-author:SantoniAA,
pubmed-author:ThiebautFF,
pubmed-author:TombesiMM,
pubmed-author:WillinghamM CMC
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
533-43
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pubmed:dateRevised |
2007-7-24
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pubmed:meshHeading |
pubmed-meshheading:1704872-Animals,
pubmed-meshheading:1704872-Antibodies, Monoclonal,
pubmed-meshheading:1704872-Antibody Specificity,
pubmed-meshheading:1704872-Cell Line,
pubmed-meshheading:1704872-Drug Resistance,
pubmed-meshheading:1704872-Epitopes,
pubmed-meshheading:1704872-Humans,
pubmed-meshheading:1704872-KB Cells,
pubmed-meshheading:1704872-Membrane Glycoproteins,
pubmed-meshheading:1704872-Mice,
pubmed-meshheading:1704872-Mice, Inbred BALB C,
pubmed-meshheading:1704872-P-Glycoprotein,
pubmed-meshheading:1704872-Precipitin Tests
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pubmed:year |
1991
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pubmed:articleTitle |
Characterization by somatic cell genetics of a monoclonal antibody to the MDR1 gene product (P-glycoprotein): determination of P-glycoprotein expression in multi-drug-resistant KB and CEM cell variants.
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pubmed:affiliation |
Laboratorio di Immunologia, Istituto Superiore di Sanità, Rome, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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