Source:http://linkedlifedata.com/resource/pubmed/id/17036057
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2007-2-6
|
| pubmed:abstractText |
Dendritic cells (DC) transfected with messenger RNA (mRNA) encoding tumor-associated antigens (TAA) are able to induce potent tumor-specific T-cell responses directed to a broad spectrum of tumor-associated epitopes. The in vitro generation of DC possessing all the features crucial for the induction of type 1 immune responses, such as mature state, migratory potential and interleukin-12 (IL-12p70) production is complicated. Particularly migratory potential is inversely correlated with IL-12p70 production after maturation with prostaglandin E2 (PGE2), which is included in maturation cocktails currently used in most vaccination trials. Here, we show that transfection of PGE2 matured DC with a single mRNA strain encoding for ubiquitin followed by a TAA which was linked to IL-12 by a self-cleaving 2A sequence, produced biological active IL-12p70 and were able to present the transfected TAA up to 72 h after transfection. Furthermore, use of the anti-reverse cap analog for in vitro transcription of the IL-12 mRNA enabled constitutive IL-12p70 production for up to 5 days. These transfected mature DC migrated efficiently towards lymph node derived chemokines. DCs constitutively expressing IL-12p70, generate TAA-specific cytotoxic T cells with an high functional avidity, independent of CD4+ T-cell help.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Cancer Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/MART-1 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/MLANA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0969-7128
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
14
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
366-75
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| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:17036057-Antigens, Neoplasm,
pubmed-meshheading:17036057-Cancer Vaccines,
pubmed-meshheading:17036057-Cells, Cultured,
pubmed-meshheading:17036057-Cytotoxicity, Immunologic,
pubmed-meshheading:17036057-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:17036057-Dendritic Cells,
pubmed-meshheading:17036057-Epitopes,
pubmed-meshheading:17036057-Gene Therapy,
pubmed-meshheading:17036057-Humans,
pubmed-meshheading:17036057-Immunophenotyping,
pubmed-meshheading:17036057-Immunotherapy, Adoptive,
pubmed-meshheading:17036057-Interleukin-12,
pubmed-meshheading:17036057-Lymphocyte Activation,
pubmed-meshheading:17036057-MART-1 Antigen,
pubmed-meshheading:17036057-Neoplasm Proteins,
pubmed-meshheading:17036057-RNA, Messenger,
pubmed-meshheading:17036057-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:17036057-Transfection
|
| pubmed:year |
2007
|
| pubmed:articleTitle |
Dendritic cells transfected with interleukin-12 and tumor-associated antigen messenger RNA induce high avidity cytotoxic T cells.
|
| pubmed:affiliation |
Department of Pathology, Vrije Universiteit Medical Center, Amsterdam, The Netherlands. hj.bontkes@vumc.nl
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|