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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2006-12-7
pubmed:abstractText
Three genetic markers of Borrelia burgdorferi have been associated with disseminated disease: the OspC type, the 16S-23S rRNA intergenic spacer type (RST), and vlsE. Here, we modified previous methods so as to identify the three markers by PCR and restriction fragment length polymorphism in parallel, analyzed B. burgdorferi isolates from erythema migrans (EM) skin lesions in 91 patients, and correlated the results with evidence of dissemination. OspC type A was found approximately twice as frequently in patients with disseminated disease, whereas type K was identified approximately twice as often in those without evidence of dissemination, but these trends were not statistically significant. The remaining seven types identified were found nearly equally in patients with or without evidence of dissemination. RST 1 strains were significantly associated with dissemination (P=0.03), whereas RST 2 and RST 3 strains tended to have an inverse association with this outcome. The vlsE gene was identified in all 91 cases, using primer sets specific for an N-terminal sequence of B. burgdorferi strain B31 (vlsEB31) or strain 297 (vlsE297), but neither marker was associated with dissemination. Specific combinations of the three genetic markers usually occurred together. OspC type A was always found with RST 1 and vlsEB31, type K was always identified with RST 2 and more often with vlsE297, and types E and I were almost always found with RST 3 and equally often with vlsEB31 and vlsE297. We conclude that B. burgdorferi strains vary in their capacity to disseminate, but almost all strains isolated from EM lesions sometimes caused disseminated disease.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-10074514, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-10377134, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-10438360, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-10566830, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-10672174, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-10678995, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-11106398, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-11119536, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-11401967, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-11450660, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-11574924, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-11705918, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-12117928, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-12198612, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-12684924, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-12694619, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-12819050, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-12874340, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-1313637, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-14605139, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-14970347, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-15184561, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-15322021, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-15514047, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-15815012, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-16049492, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-16079876, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-16381837, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-2231712, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-3662285, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-7598144, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-7751362, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-8727927, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-9108482, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-9403685, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-9514637, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-9673250, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-9673251, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-9872945, http://linkedlifedata.com/resource/pubmed/commentcorrection/17035489-9986813
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4407-13
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:17035489-Antigens, Bacterial, pubmed-meshheading:17035489-Bacterial Outer Membrane Proteins, pubmed-meshheading:17035489-Bacterial Proteins, pubmed-meshheading:17035489-Biological Markers, pubmed-meshheading:17035489-Borrelia burgdorferi, pubmed-meshheading:17035489-DNA, Bacterial, pubmed-meshheading:17035489-DNA, Ribosomal Spacer, pubmed-meshheading:17035489-Gene Frequency, pubmed-meshheading:17035489-Genes, Bacterial, pubmed-meshheading:17035489-Genotype, pubmed-meshheading:17035489-Humans, pubmed-meshheading:17035489-Lipoproteins, pubmed-meshheading:17035489-Lyme Disease, pubmed-meshheading:17035489-Polymerase Chain Reaction, pubmed-meshheading:17035489-Polymorphism, Restriction Fragment Length, pubmed-meshheading:17035489-Skin, pubmed-meshheading:17035489-Statistics as Topic, pubmed-meshheading:17035489-Virulence
pubmed:year
2006
pubmed:articleTitle
Borrelia burgdorferi genetic markers and disseminated disease in patients with early Lyme disease.
pubmed:affiliation
Division of Rheumatology, Allergy, and Immunology, Center for Immunology and Inflammatory Disease, Harvard Medical School, Massachusetts General Hospital, 55 Fruit St., CNY 149/8301, Boston, MA 02114, USA. KLJones@partners.org
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