Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2006-10-11
pubmed:abstractText
Protein-protein interactions are required for many viral and cellular functions and are potential targets for novel therapies. Here we detail a series of genetic and biochemical techniques used in combination to find an essential molecular contact point on the duck hepatitis B virus polymerase. These techniques include differential immunoprecipitation, mutagenesis and peptide competition. The strength of these techniques is their ability to identify contact points on intact proteins or protein complexes employing functional assays. This approach can be used to aid identification of putative binding sites on proteins and protein complexes which are resistant to characterization by other methods.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-10091594, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-10455170, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-10954566, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-11689048, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-11739692, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-12125015, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-12851401, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-12857903, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-15064463, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-15231674, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-15308704, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-15585172, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-15880642, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-15882193, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-15939335, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-15993577, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-16051809, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-16337697, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-1874717, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-2023948, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-3012360, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-7520092, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-7692081, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-8189492, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-8709189, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-9392700, http://linkedlifedata.com/resource/pubmed/commentcorrection/17033698-9621038
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:issn
1480-9222
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
77-86
pubmed:dateRevised
2010-9-16
pubmed:year
2006
pubmed:articleTitle
Combining genetic and biochemical approaches to identify functional molecular contact points.
pubmed:affiliation
Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine, St. Louis, MO 63104, USA.
pubmed:publicationType
Journal Article