Linked Life Data

17033082

Source:http://linkedlifedata.com/resource/pubmed/id/17033082

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2006-10-11
pubmed:abstractText
A better understanding of fungal biology will facilitate judicious use of beneficial fungi and will also advance our efforts to control pathogenic fungi. Molecular studies of fungal biology have been greatly aided by transformation-mediated mutagenesis techniques. Transformation via nonhomologous integration of plasmid DNA bearing a selectable marker (e.g., antibiotic resistance gene) has been widely used for the random insertional mutagenesis of fungi - as an alternative to chemical and radiation mutagens - mainly because the integration of plasmid into the genome provides a convenient tag for subsequent identification and isolation of the mutated gene. Homologous recombination between a target gene on the chromosome and the introduced DNA carrying its mutant allele results in targeted gene knock-out. An important advance in fungal transformation methodology is the development of Agrobacterium tumefaciens-mediated transformation (ATMT) protocols for fungi. ATMT has been successfully applied to a phylogenetically diverse group of fungi and offers a number of advantages over conventional transformation techniques in both the random insertional mutagenesis and targeted gene knock-out. In this chapter, we describe ATMT protocols and vectors for fungal gene manipulation using two plant pathogenic fungi, Magnaporthe grisea and Fusarium oxysporum, as target organisms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
344
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
403-20
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Filamentous Fungi (Magnaporthe grisea and Fusarium oxysporum).
pubmed:affiliation
Department of Plant Pathology, The Pennsylvania State University, University Park, PA, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't