| pubmed-article:1703160 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1703160 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:1703160 | lifeskim:mentions | umls-concept:C1167395 | lld:lifeskim |
| pubmed-article:1703160 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:1703160 | lifeskim:mentions | umls-concept:C0029073 | lld:lifeskim |
| pubmed-article:1703160 | lifeskim:mentions | umls-concept:C1158478 | lld:lifeskim |
| pubmed-article:1703160 | lifeskim:mentions | umls-concept:C1705422 | lld:lifeskim |
| pubmed-article:1703160 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:1703160 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:1703160 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:1703160 | pubmed:dateCreated | 1991-2-27 | lld:pubmed |
| pubmed-article:1703160 | pubmed:abstractText | The structural genes of the ilvGMEDA operon of Escherichia coli are preceded by two promoters, ilvPG1 and ilvPG2, and a leader-attenuator region. Alkylation protection and hydroxyl radical footprinting techniques have been used to demonstrate that integration host factor (IHF) interacts with the nucleotides in a consensus-like DNA sequence located immediately downstream of the RNA polymerase transcriptional pause site in the leader-attenuator region. In the presence of purified IHF protein, in vitro transcriptional pausing of RNA polymerase at the leader-attenuator pause site is increased 2-fold and, concomitantly, a 2-fold increase in transcriptional termination at the attenuator is observed. Strains containing chromosomal transcriptional fusions of various segments of the ilvGMEDA promoter-attenuator region to the galK gene were used to show that IHF also decreases the in vivo basal level of transcriptional readthrough at the attenuator 2-fold. The binding of IHF to another target site in the ilvPG1 promoter region represses transcription from this promoter and causes a 4-fold stimulation of transcription initiation from the downstream ilvPG2 promoter 4-fold. This IHF-mediated control of transcription initiation from the upstream promoter region is independent of the regulation of transcription termination effected by IHF interaction at the attenuator site. Thus, IHF is capable of regulating the expression of the ilvGMEDA operon in opposing manners; it can activate transcription initiation of this operon from the ilvPG2 promoter 4-fold and increase the termination of this transcription at the downstream attenuator 2-fold. | lld:pubmed |
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| pubmed-article:1703160 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:1703160 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1703160 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1703160 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:1703160 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:1703160 | pubmed:author | pubmed-author:HatfieldG WGW | lld:pubmed |
| pubmed-article:1703160 | pubmed:author | pubmed-author:PagelJ MJM | lld:pubmed |
| pubmed-article:1703160 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1703160 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:1703160 | pubmed:volume | 266 | lld:pubmed |
| pubmed-article:1703160 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1703160 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1703160 | pubmed:pagination | 1985-96 | lld:pubmed |
| pubmed-article:1703160 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:1703160 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1703160 | pubmed:articleTitle | Integration host factor-mediated expression of the ilvGMEDA operon of Escherichia coli. | lld:pubmed |
| pubmed-article:1703160 | pubmed:affiliation | Department of Microbiology and Molecular Genetics, California College of Medicine, University of California, Irvine 92717. | lld:pubmed |
| pubmed-article:1703160 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1703160 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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