Linked Life Data

1703160

Source:http://linkedlifedata.com/resource/pubmed/id/1703160

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1991-2-27
pubmed:abstractText
The structural genes of the ilvGMEDA operon of Escherichia coli are preceded by two promoters, ilvPG1 and ilvPG2, and a leader-attenuator region. Alkylation protection and hydroxyl radical footprinting techniques have been used to demonstrate that integration host factor (IHF) interacts with the nucleotides in a consensus-like DNA sequence located immediately downstream of the RNA polymerase transcriptional pause site in the leader-attenuator region. In the presence of purified IHF protein, in vitro transcriptional pausing of RNA polymerase at the leader-attenuator pause site is increased 2-fold and, concomitantly, a 2-fold increase in transcriptional termination at the attenuator is observed. Strains containing chromosomal transcriptional fusions of various segments of the ilvGMEDA promoter-attenuator region to the galK gene were used to show that IHF also decreases the in vivo basal level of transcriptional readthrough at the attenuator 2-fold. The binding of IHF to another target site in the ilvPG1 promoter region represses transcription from this promoter and causes a 4-fold stimulation of transcription initiation from the downstream ilvPG2 promoter 4-fold. This IHF-mediated control of transcription initiation from the upstream promoter region is independent of the regulation of transcription termination effected by IHF interaction at the attenuator site. Thus, IHF is capable of regulating the expression of the ilvGMEDA operon in opposing manners; it can activate transcription initiation of this operon from the ilvPG2 promoter 4-fold and increase the termination of this transcription at the downstream attenuator 2-fold.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
266
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1985-96
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:1703160-Amino Acids, Branched-Chain, pubmed-meshheading:1703160-Bacterial Proteins, pubmed-meshheading:1703160-Base Sequence, pubmed-meshheading:1703160-DNA, Bacterial, pubmed-meshheading:1703160-DNA-Binding Proteins, pubmed-meshheading:1703160-DNA-Directed RNA Polymerases, pubmed-meshheading:1703160-Escherichia coli, pubmed-meshheading:1703160-Gene Expression Regulation, Bacterial, pubmed-meshheading:1703160-Hydroxides, pubmed-meshheading:1703160-Integration Host Factors, pubmed-meshheading:1703160-Kinetics, pubmed-meshheading:1703160-Molecular Sequence Data, pubmed-meshheading:1703160-Operon, pubmed-meshheading:1703160-Promoter Regions, Genetic, pubmed-meshheading:1703160-RNA, Bacterial, pubmed-meshheading:1703160-RNA, Messenger, pubmed-meshheading:1703160-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:1703160-Transcription, Genetic
pubmed:year
1991
pubmed:articleTitle
Integration host factor-mediated expression of the ilvGMEDA operon of Escherichia coli.
pubmed:affiliation
Department of Microbiology and Molecular Genetics, California College of Medicine, University of California, Irvine 92717.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.