Linked Life Data

17029715

Source:http://linkedlifedata.com/resource/pubmed/id/17029715

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2006-10-10
pubmed:abstractText
A fast perturbation in proton concentration can be induced in aqueous solution using a pulsed ultraviolet laser and suitable photolabile compounds which, upon photoexcitation, irreversibly release protons. The volume change and the rate constant for the reaction of the photodetached protons with proton-accepting groups in solution can be monitored using time resolved photoacoustics. A typical proton concentration jump of 1 microM can be obtained with a 200-microJ laser pulse at 308 nm. Reaction dynamics from 20 ns to 5 micros can be easily followed. The methodology we establish represents a direct, time-resolved measurement of the reaction volume in proton transfer processes and an extension to the nanosecond-microsecond range of traditional relaxation techniques, such as stopped-flow. We report example applications to reactions involving simple molecules and polypeptides.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Jul
pubmed:issn
0301-4622
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
13-22
pubmed:year
1998
pubmed:articleTitle
An experimental methodology for measuring volume changes in proton transfer reactions in aqueous solutions.
pubmed:affiliation
Istituto Nazionale per la Fisica della Materia, Parma, Italy. viappiani@vaxpr.fis.unipr.it
pubmed:publicationType
Journal Article