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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2007-1-8
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pubmed:abstractText |
Inhalation of particulate matter aggravates respiratory symptoms in patients with chronic airway diseases, but the mechanisms underlying this response remain poorly understood. We used a proteomics approach to examine this phenomenon. Treatment of epithelial cells with BSA-coated titanium dioxide (TiO(2)) particles altered 20 protein spots on the two-dimensional gel, and these were then analyzed by nano-LC-MS/MS. These proteins included defense-related, cell-activating, and cytoskeletal proteins implicated in the response to oxidative stress. The proteins were classified into four groups according to the time course of their expression patterns. For validation, RT-PCR was performed on extracts of in vitro TiO(2)-treated cells, and lung issues from TiO(2)-treated rats were analyzed by immunohistochemical staining and enzyme immunoassay. TiO(2) treatment was found to increase the amount of mRNA for macrophage migration-inhibitory factor (MIF). MIF was expressed primarily in epithelium and was elevated in lung tissues and bronchoalveolar lavage fluids of TiO(2)-treated rats as compared with sham-treated rats. Carbon black and diesel exhaust particles also induced expression of MIF protein in the epithelial cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1535-9476
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
56-63
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pubmed:meshHeading |
pubmed-meshheading:17028300-Amino Acid Sequence,
pubmed-meshheading:17028300-Animals,
pubmed-meshheading:17028300-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:17028300-Chloride Channels,
pubmed-meshheading:17028300-Cluster Analysis,
pubmed-meshheading:17028300-Cytoplasm,
pubmed-meshheading:17028300-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:17028300-Epithelial Cells,
pubmed-meshheading:17028300-Gene Expression Regulation,
pubmed-meshheading:17028300-Humans,
pubmed-meshheading:17028300-Lung,
pubmed-meshheading:17028300-Macrophage Migration-Inhibitory Factors,
pubmed-meshheading:17028300-Male,
pubmed-meshheading:17028300-Molecular Sequence Data,
pubmed-meshheading:17028300-Particulate Matter,
pubmed-meshheading:17028300-Proteomics,
pubmed-meshheading:17028300-RNA, Messenger,
pubmed-meshheading:17028300-Rats,
pubmed-meshheading:17028300-Rats, Sprague-Dawley,
pubmed-meshheading:17028300-Titanium,
pubmed-meshheading:17028300-Transaldolase
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pubmed:year |
2007
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pubmed:articleTitle |
Proteomic identification of macrophage migration-inhibitory factor upon exposure to TiO2 particles.
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pubmed:affiliation |
Genome Research Center for Allergy and Respiratory Diseases, Soonchunhyang University Hospital, Bucheon-si, Gyeonggi-do 420-853, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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