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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1991-2-13
pubmed:databankReference
pubmed:abstractText
Tumor necrosis factor-alpha (TNF-alpha), a protein released by activated macrophages, is involved in a wide variety of human diseases including septic shock, cachexia, and chronic inflammation. TNF binding protein (TNF-BP), a glycoprotein with high affinity to TNF-alpha isolated from urine, acts as an inhibitor of TNF-alpha by competing with the cell-surface TNF receptor. We report here the partial amino acid sequencing of human TNF-BP as well as the isolation, sequence, and expression of cDNA clones encoding a human and rat TNF receptor. The calculated Mr of the mature human and rat TNF receptor chains is 47,526 and 48,072, respectively. The extracellular ligand binding domain represents the soluble TNF-BP which is released by proteolytic cleavage. TNF-BP contains 24 cysteine residues and three potential N-glycosylation sites and shows sequence homology to the extracellular portions of TNF-R p80 chain and nerve growth factor receptor. Transfection of the human TNF receptor cDNA into mammalian cells resulted in increased binding capacity for TNF-alpha and increased reactivity with a monoclonal antibody directed against the human TNF receptor chain p60. When a stop codon was introduced into the cDNA at the site corresponding to the carboxyl terminus of TNF-BP, transfected cells secreted a protein that reacted with antibodies raised against natural TNF-BP.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1044-5498
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
705-15
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1702293-Amino Acid Sequence, pubmed-meshheading:1702293-Animals, pubmed-meshheading:1702293-Base Sequence, pubmed-meshheading:1702293-Blotting, Northern, pubmed-meshheading:1702293-Cloning, Molecular, pubmed-meshheading:1702293-DNA, pubmed-meshheading:1702293-Gene Expression, pubmed-meshheading:1702293-Humans, pubmed-meshheading:1702293-Molecular Sequence Data, pubmed-meshheading:1702293-Plasmids, pubmed-meshheading:1702293-Poly A, pubmed-meshheading:1702293-Polymerase Chain Reaction, pubmed-meshheading:1702293-RNA, pubmed-meshheading:1702293-RNA, Messenger, pubmed-meshheading:1702293-Rats, pubmed-meshheading:1702293-Receptors, Cell Surface, pubmed-meshheading:1702293-Receptors, Nerve Growth Factor, pubmed-meshheading:1702293-Receptors, Tumor Necrosis Factor, pubmed-meshheading:1702293-Transfection, pubmed-meshheading:1702293-Tumor Necrosis Factor-alpha
pubmed:year
1990
pubmed:articleTitle
Molecular cloning and expression of human and rat tumor necrosis factor receptor chain (p60) and its soluble derivative, tumor necrosis factor-binding protein.
pubmed:affiliation
Ernst Boehringer Institut, Bender + Co GesmbH, Vienna, Austria.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't