Linked Life Data

17018627

Source:http://linkedlifedata.com/resource/pubmed/id/17018627

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
2006-10-4
pubmed:abstractText
Fibrinogen is a major plasma protein (350 kDa) that induces proliferative signals by serving as a scaffold to support the binding of growth factors and to promote the cellular responses of adhesion, proliferation, and migration during wound healing, angiogenesis, and tumor growth. Fibrin(ogen) degradation products generated during fibrinolysis are implicated in tissue injury. The fibrinogen gamma chain has a COOH-terminal globular domain (gamma C, residues 151-411 of the gamma chain, 30 kDa) to which several integrin cell adhesion receptors (e.g., platelet alpha(IIb)beta(3), endothelial alpha(v)beta(3), and leukocyte alpha(M)beta(2)) bind. Integrins play a critical role in signal transduction from fibrin(ogen). We found that gamma C and its truncation mutant (designated gamma C399tr), with a deletion of the COOH-terminal 12 residues, induced apoptosis of endothelial cells and blocked tube formation of endothelial cells. DLD-1 human colon cancer cells that secrete gamma C or gamma C399tr grew at similar levels in vitro but grew much slower in vivo than mock-transfected cells. The recombinant purified gamma C399tr fragment markedly suppressed tumor growth, development of intratumoral vasculature, and tumor metastasis in vivo in the highly metastatic Met-1 breast cancer model. The determinant responsible for binding to endothelial cells is cryptic in native fibrinogen but is exposed in gamma C and gamma C399tr. These results suggest that fibrinogen has a novel cryptic determinant, which can exert apoptosis-inducing activity on endothelial cells when exposed, and polypeptides containing this determinant have therapeutic potential.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1538-7445
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9691-7
pubmed:dateRevised
2007-12-3
pubmed:meshHeading
pubmed-meshheading:17018627-Angiogenesis Inhibitors, pubmed-meshheading:17018627-Animals, pubmed-meshheading:17018627-Antineoplastic Agents, pubmed-meshheading:17018627-Apoptosis, pubmed-meshheading:17018627-Cells, Cultured, pubmed-meshheading:17018627-Colonic Neoplasms, pubmed-meshheading:17018627-Endothelial Cells, pubmed-meshheading:17018627-Fibrin Fibrinogen Degradation Products, pubmed-meshheading:17018627-Fibrinogen, pubmed-meshheading:17018627-Humans, pubmed-meshheading:17018627-Integrin alphaVbeta3, pubmed-meshheading:17018627-Lung Neoplasms, pubmed-meshheading:17018627-Mammary Neoplasms, Experimental, pubmed-meshheading:17018627-Mice, pubmed-meshheading:17018627-Mice, Nude, pubmed-meshheading:17018627-Mice, SCID, pubmed-meshheading:17018627-Morphogenesis, pubmed-meshheading:17018627-Mutation, pubmed-meshheading:17018627-Peptide Fragments, pubmed-meshheading:17018627-Protein Binding, pubmed-meshheading:17018627-Protein Structure, Tertiary, pubmed-meshheading:17018627-Recombinant Fusion Proteins, pubmed-meshheading:17018627-Sequence Deletion, pubmed-meshheading:17018627-Transfection
pubmed:year
2006
pubmed:articleTitle
The COOH-terminal globular domain of fibrinogen gamma chain suppresses angiogenesis and tumor growth.
pubmed:affiliation
Department of Dermatology, University of California-Davis Medical Center, Sacramento, CA 95817, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural