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rdf:type |
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lifeskim:mentions |
umls-concept:C0004561,
umls-concept:C0020846,
umls-concept:C0021758,
umls-concept:C0086418,
umls-concept:C0205250,
umls-concept:C0205263,
umls-concept:C0220781,
umls-concept:C0599739,
umls-concept:C1519138,
umls-concept:C1539081,
umls-concept:C1883254,
umls-concept:C1998793
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pubmed:issue |
6
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pubmed:dateCreated |
1991-1-31
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pubmed:abstractText |
A novel pathway of IgE-B cell differentiation has been identified. Engagement of the B cell antigen CD40 by F(ab')2 fragments of monoclonal antibody (mAb) 626.1 in the presence of recombinant interleukin 4 (rIL-4) induced intense IgE synthesis, but modest IgG synthesis, by highly purified human B cells. Surface IgE- B cells isolated by cell sorting were induced to produce IgE by mAb 626.1 and IL-4. Thus, IgE synthesis is unlikely to result from expansion of a B cell population precommitted to IgE in vivo. A neutralizing anti-IL-6 antibody strongly, but not completely, inhibited the IgE response. This indicates that autocrine production of IL-6 plays an important amplification role in IgE synthesis triggered by anti-CD40 mAb and IL-4. Although the exact role played by CD40 in IgE responses in vivo remains to be established, this T cell-independent system represents a useful model to characterize the biochemical and molecular events leading to IgE synthesis in human B cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-1695667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-1696597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-2106137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-2110192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-2136895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-2137840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-2160731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-2470814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-2475341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-2480376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-2522501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-2536066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-2546158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-2654173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-2677211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-2789139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-2813375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1701824-3492534
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Fab Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
172
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1861-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1701824-Antibodies, Monoclonal,
pubmed-meshheading:1701824-Antigens, CD,
pubmed-meshheading:1701824-Antigens, CD40,
pubmed-meshheading:1701824-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:1701824-B-Lymphocytes,
pubmed-meshheading:1701824-Cells, Cultured,
pubmed-meshheading:1701824-Flow Cytometry,
pubmed-meshheading:1701824-Humans,
pubmed-meshheading:1701824-Immunoglobulin E,
pubmed-meshheading:1701824-Immunoglobulin Fab Fragments,
pubmed-meshheading:1701824-Interleukin-4,
pubmed-meshheading:1701824-Interleukin-6,
pubmed-meshheading:1701824-Recombinant Proteins
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pubmed:year |
1990
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pubmed:articleTitle |
CD40 and IgE: synergism between anti-CD40 monoclonal antibody and interleukin 4 in the induction of IgE synthesis by highly purified human B cells.
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pubmed:affiliation |
Children's Hospital, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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