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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2006-10-10
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pubmed:abstractText |
Neuregulin1 (NRG1) is a growth factor that signals through the interaction of the epidermal growth factor (EGF)-like domain with ErbB receptors. An immunoglobulin (Ig)-like domain is contained together with EGF-like domain in the ectodomain of some isoforms generated by alternative splicing, but its role in NRG1 signaling remained unclear. In the present study, we identified a novel isoform of NRG1 containing an Ig-like domain conserved among species from adult Xenopus laevis, which is predominantly expressed in the testis and brain. We generated recombinant proteins for the whole ectodomain and EGF-like domain alone of the isoform to compare their effects on cell proliferation, and phosphorylation of and their association with ErbB receptor, demonstrating that the ectodomain had approximately 10(3)-fold higher abilities than the EGF-like domain. Therefore, the Ig-like domain is probably essential for efficient interaction of an EGF-like domain with ErbB receptors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
350
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
263-71
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pubmed:dateRevised |
2011-11-2
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pubmed:meshHeading |
pubmed-meshheading:17007820-Amino Acid Sequence,
pubmed-meshheading:17007820-Animals,
pubmed-meshheading:17007820-Brain,
pubmed-meshheading:17007820-Cell Line, Tumor,
pubmed-meshheading:17007820-Cell Proliferation,
pubmed-meshheading:17007820-Cloning, Molecular,
pubmed-meshheading:17007820-Immunoglobulins,
pubmed-meshheading:17007820-Male,
pubmed-meshheading:17007820-Mice,
pubmed-meshheading:17007820-Molecular Sequence Data,
pubmed-meshheading:17007820-Neuregulin-1,
pubmed-meshheading:17007820-Protein Isoforms,
pubmed-meshheading:17007820-Protein Structure, Tertiary,
pubmed-meshheading:17007820-RNA, Messenger,
pubmed-meshheading:17007820-Receptor, Epidermal Growth Factor,
pubmed-meshheading:17007820-Sequence Alignment,
pubmed-meshheading:17007820-Testis,
pubmed-meshheading:17007820-Tissue Distribution,
pubmed-meshheading:17007820-Xenopus Proteins,
pubmed-meshheading:17007820-Xenopus laevis
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pubmed:year |
2006
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pubmed:articleTitle |
The immunoglobulin-like domain is involved in interaction of Neuregulin1 with ErbB.
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pubmed:affiliation |
Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan. etoko@gpo.kumamoto-u.ac.jp
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pubmed:publicationType |
Journal Article
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