Linked Life Data

16980429

Source:http://linkedlifedata.com/resource/pubmed/id/16980429

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2006-11-7
pubmed:abstractText
The sensitivity of a test for cattle shedding Escherichia coli serogroup O26 was estimated using several fecal pats artificially inoculated at a range of concentrations with different E. coli O26 strains. The test involves the enrichment of fecal microflora in buffered peptone water, the selective concentration of E. coli O26 using antibody-coated immunomagnetic-separation beads, the identification of E. coli colonies on Chromocult tryptone bile X-glucuronide agar, and confirmation of the serogroup with E. coli serogroup O26-specific antisera using slide agglutination. The effective dose of E. coli O26 for an 80% test sensitivity (ED(80)) was 1.0 x 10(4) CFU g(-1) feces (95% confidence interval, 4.7 x 10(3) to 2.4 x 10(4)). Differences in test sensitivity between different E. coli O26 strains and fecal pats were also observed. Individual estimates of ED(80) for each strain and fecal pat combination ranged from 4.2 x 10(2) to 4.8 x 10(5) CFU g(-1). These results suggest that the test is useful for identifying individuals shedding a large number of E. coli O26 organisms or, if an appropriate number of individuals in a herd are sampled, for identifying affected herds. The study also provides a benchmark estimate of sensitivity that can be used to compare alternative tests for E. coli O26 and a methodological approach that can be applied to tests for other pathogenic members of the Enterobacteriaceae and other sample types.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16980429-10543842, http://linkedlifedata.com/resource/pubmed/commentcorrection/16980429-10665540, http://linkedlifedata.com/resource/pubmed/commentcorrection/16980429-11142505, http://linkedlifedata.com/resource/pubmed/commentcorrection/16980429-11880417, http://linkedlifedata.com/resource/pubmed/commentcorrection/16980429-12233845, http://linkedlifedata.com/resource/pubmed/commentcorrection/16980429-12904221, http://linkedlifedata.com/resource/pubmed/commentcorrection/16980429-14933043, http://linkedlifedata.com/resource/pubmed/commentcorrection/16980429-14979592, http://linkedlifedata.com/resource/pubmed/commentcorrection/16980429-15006796, http://linkedlifedata.com/resource/pubmed/commentcorrection/16980429-15336421, http://linkedlifedata.com/resource/pubmed/commentcorrection/16980429-16391103, http://linkedlifedata.com/resource/pubmed/commentcorrection/16980429-16517637, http://linkedlifedata.com/resource/pubmed/commentcorrection/16980429-2056066, http://linkedlifedata.com/resource/pubmed/commentcorrection/16980429-3886804, http://linkedlifedata.com/resource/pubmed/commentcorrection/16980429-9212405, http://linkedlifedata.com/resource/pubmed/commentcorrection/16980429-9466788
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7260-3
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Sensitivity of an immunomagnetic-separation-based test for detecting Escherichia coli O26 in bovine feces.
pubmed:affiliation
Scottish Agricultural College, Stratherrick Rd., Inverness IV2 4JZ, United Kingdom. malcolm.hall@sac.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies