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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1990-10-18
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pubmed:databankReference |
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pubmed:abstractText |
The Ly-6E/A antigen is expressed on activated murine T cells. Using probes made from the previously characterized cDNA, we have isolated a genomic DNA clone encoding the Ly-6A antigen. We determined the DNA sequence of the genomic clone and conducted a functional analysis of the promoter region. Mouse fibroblast BALB/3T3 cells transfected with this genomic clone constitutively expressed Ly-6A antigen on their cell surface. This expression was inducible by alpha/beta and gamma interferons. The Ly-6E 5'-flanking region was analyzed by chloramphenicol acetyltransferase assays in fibroblast cells for cis-acting elements. At least two positive elements were found to be needed for maximum constitutive promoter activity in L cells. One of the positive elements was specifically bound by a CCAAT box-binding protein from crude nuclear extract, as shown by electrophoretic mobility shift assays and footprinting. The other element, which contains a GGAAA motif and has homology to various known enhancers, also showed a specific binding activity. This second positive element when multimerized became a very powerful enhancing element. Interferon treatment could enhance expression of the chloramphenicol acetyltransferase gene fused to the Ly-6E 5'-flanking region in stably transfected BALB/3T3 cells. The elements responsible for this enhancement lie, at least in part, between positions -1760 and -900 of the gene. Surprisingly, there is no sequence homology between this region of Ly-6E and the established consensus for the interferon-stimulated response element, which has been shown functionally important to all previously characterized alpha/beta interferon-inducible promoters. The Ly-6E gene may prove to be a novel system for the study of interferon induction.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-2474447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-2660142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-2697681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-2796989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-2822573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-2823261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-2845412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-2894252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-2895473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-2898810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-2922289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-2987671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-3015413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-3028776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-3141929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-3349524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-3356904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-3359997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-3372536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-3399893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-3476205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-3489060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-3561391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-3990797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-6275366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-6336593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-6828386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1697928-6960240
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5150-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1697928-Amino Acid Sequence,
pubmed-meshheading:1697928-Animals,
pubmed-meshheading:1697928-Antigens, Ly,
pubmed-meshheading:1697928-Base Sequence,
pubmed-meshheading:1697928-Cloning, Molecular,
pubmed-meshheading:1697928-DNA Mutational Analysis,
pubmed-meshheading:1697928-DNA-Binding Proteins,
pubmed-meshheading:1697928-Gene Expression Regulation,
pubmed-meshheading:1697928-Genes,
pubmed-meshheading:1697928-Hematopoietic Stem Cells,
pubmed-meshheading:1697928-Interferons,
pubmed-meshheading:1697928-L Cells (Cell Line),
pubmed-meshheading:1697928-Mice,
pubmed-meshheading:1697928-Molecular Sequence Data,
pubmed-meshheading:1697928-Promoter Regions, Genetic,
pubmed-meshheading:1697928-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1697928-Restriction Mapping,
pubmed-meshheading:1697928-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1697928-T-Lymphocytes,
pubmed-meshheading:1697928-Transfection
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pubmed:year |
1990
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pubmed:articleTitle |
Characterization of promoter elements of an interferon-inducible Ly-6E/A differentiation antigen, which is expressed on activated T cells and hematopoietic stem cells.
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pubmed:affiliation |
Department of Biology, Yale University Medical School, New Haven, Connecticut 06510.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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