Linked Life Data

1696871

Source:http://linkedlifedata.com/resource/pubmed/id/1696871

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-9-27
pubmed:abstractText
Lymphocyte function and cell surface phenotype were examined in fifteen patients with late onset hypogammaglobulinaemia. The percentage of surface immunoglobulin-positive B cells in fourteen of the fifteen patients was in the normal range. Patients' B cells expressed MHC class II antigens at normal levels. For one patient, there was relatively high sIgD and low sIgM expression on B cells; the rest of the patients did not differ from controls in surface immunoglobulin density. The proportion of B cells positive for CD5 in patients was comparable to normal controls, and considerably less than in cord blood. However, the pattern of immunoglobulin isotype secretion in vitro by patients' B cells closely paralleled responses of cord blood B cells. Spontaneous secretion of IgM and IgG by patients' B cells was very low. Following polyclonal activation in the presence of autologous T cells, cells from thirteen patients secreted IgM within the normal range in response to at least one activator. The response of patients' purified B cells to IL-2 and gamma-IFN was variable. For four of six tested, B cells cultured with IL-2 and gamma-IFN together with polyclonal activators secreted normal levels of IgM. B cells from the other two patients secreted little or no IgM in response to these cytokines. For fourteen patients, IgG secretion following polyclonal activation remained low both when B cells were cultured with T cells or with a combination of IL-2 and gamma-IFN. IgG subclass imbalance was seen in one patient, whose cells secreted an unusually high proportion of IgG3, and undetectable IgG2 and IgG4; this pattern was consistent whether T cell help was provided by autologous or allogeneic T cells. Similarly purified B cells from this patient showed deficient IgG2 and IgG4 production in response to IL-2 and gamma-IFN.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0278-0240
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
69-83
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:1696871-Adult, pubmed-meshheading:1696871-Agammaglobulinemia, pubmed-meshheading:1696871-Aged, pubmed-meshheading:1696871-Antigens, CD, pubmed-meshheading:1696871-Antigens, CD5, pubmed-meshheading:1696871-Antigens, Differentiation, pubmed-meshheading:1696871-B-Lymphocytes, pubmed-meshheading:1696871-Cell Membrane, pubmed-meshheading:1696871-Female, pubmed-meshheading:1696871-HLA-DR Antigens, pubmed-meshheading:1696871-Histocompatibility Antigens Class II, pubmed-meshheading:1696871-Humans, pubmed-meshheading:1696871-Immunoglobulin G, pubmed-meshheading:1696871-Immunoglobulin M, pubmed-meshheading:1696871-Interferon-gamma, pubmed-meshheading:1696871-Interleukin-2, pubmed-meshheading:1696871-Lymphocyte Activation, pubmed-meshheading:1696871-Male, pubmed-meshheading:1696871-Middle Aged, pubmed-meshheading:1696871-Phenotype, pubmed-meshheading:1696871-Recombinant Proteins, pubmed-meshheading:1696871-T-Lymphocytes
pubmed:articleTitle
B cell differentiation and lymphocyte surface phenotype in late onset hypogammaglobulinaemia.
pubmed:affiliation
Division of Immunology, School of Pathological Sciences, University of Newcastle upon Tyne.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't