Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1990-7-13
|
| pubmed:abstractText |
Galanin is a widely distributed regulatory peptide which modulates the pituitary secretion of PRL and GH. Estrogen administration strongly stimulates galanin gene expression in the rat anterior pituitary. In adult female Fischer 344 rats, estrogen also induces hyperplasia of lactotropes. We used immunocytochemical analysis to assess the effects of estrogen on galanin-like immunoreactivity (Gal-IR) in the rat pituitary and hypothalamus during sc diethylstilbestrol (DES) implantation and after its removal at 30 days. In the anterior pituitary, DES implantation increased the portion of Gal-IR-containing cells from less than 2% in the control rats to 18.3% after 3 days of DES and 36% after 30 days. These changes paralleled the lactotrope hyperplasia exhibited in response to DES exposure. Ten and 30 days after removal of the DES capsules, the percentage of Gal-IR-containing cells in the anterior pituitary decreased to 6.3% and 1.5%, respectively. Colocalization studies revealed that Gal-IR-containing cells were predominantly lactotropes. Immunoelectron microscopy demonstrated that Gal-IR was concentrated in the Golgi region of these hyperplastic lactotropes and suggests that little of the synthesized galanin is secreted. The distribution of Gal-IR in the hypothalamus, median eminence, and neurohypophysis was unaffected by DES treatment. These data demonstrate that galanin is synthesized by hyperplastic pituitary lactotropes of Fischer 344 rats and that peptide accumulation is dependent on the presence of circulating estrogens. In contrast, neuronal galanin synthesis in the hypothalamus does not appear to be regulated by estrogen.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Diethylstilbestrol,
http://linkedlifedata.com/resource/pubmed/chemical/Galanin,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Prolactin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0013-7227
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
126
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3159-67
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1693569-Animals,
pubmed-meshheading:1693569-Cytoplasmic Granules,
pubmed-meshheading:1693569-Diethylstilbestrol,
pubmed-meshheading:1693569-Female,
pubmed-meshheading:1693569-Galanin,
pubmed-meshheading:1693569-Golgi Apparatus,
pubmed-meshheading:1693569-Growth Hormone,
pubmed-meshheading:1693569-Hyperplasia,
pubmed-meshheading:1693569-Hypothalamus,
pubmed-meshheading:1693569-Immunoenzyme Techniques,
pubmed-meshheading:1693569-Median Eminence,
pubmed-meshheading:1693569-Microscopy, Electron,
pubmed-meshheading:1693569-Peptides,
pubmed-meshheading:1693569-Pituitary Gland, Anterior,
pubmed-meshheading:1693569-Pituitary Gland, Posterior,
pubmed-meshheading:1693569-Prolactin,
pubmed-meshheading:1693569-Rats,
pubmed-meshheading:1693569-Rats, Inbred F344,
pubmed-meshheading:1693569-Tissue Distribution
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Estrogen increases galanin immunoreactivity in hyperplastic prolactin-secreting cells in Fisher 344 rats.
|
| pubmed:affiliation |
C.S. Kubik Laboratory for Neuropathology, Massachusetts General Hospital, Boston.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|