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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2006-9-6
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pubmed:abstractText |
Whole genome amplification is an increasingly common technique through which minute amounts of DNA can be multiplied to generate quantities suitable for genetic testing and analysis. Questions of amplification-induced error and template bias generated by these methods have previously been addressed through either small scale (SNPs) or large scale (CGH array, FISH) methodologies. Here we utilized whole genome sequencing to assess amplification-induced bias in both coding and non-coding regions of two bacterial genomes. Halobacterium species NRC-1 DNA and Campylobacter jejuni were amplified by several common, commercially available protocols: multiple displacement amplification, primer extension pre-amplification and degenerate oligonucleotide primed PCR. The amplification-induced bias of each method was assessed by sequencing both genomes in their entirety using the 454 Sequencing System technology and comparing the results with those obtained from unamplified controls.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16928277-10471496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16928277-10631774,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/16928277-11959976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16928277-12220898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16928277-12566408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16928277-12695328,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/16928277-14576329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16928277-14624861,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/16928277-1639399,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/16928277-1848217,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/16928277-8593063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16928277-8787693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16928277-8962113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16928277-9662393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16928277-9758791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16928277-9916922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16928277-9927758
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1471-2164
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
216
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:16928277-Bias (Epidemiology),
pubmed-meshheading:16928277-Campylobacter jejuni,
pubmed-meshheading:16928277-Chromosomes, Bacterial,
pubmed-meshheading:16928277-DNA Probes,
pubmed-meshheading:16928277-Genome, Bacterial,
pubmed-meshheading:16928277-Genomics,
pubmed-meshheading:16928277-Halobacterium,
pubmed-meshheading:16928277-Nucleic Acid Amplification Techniques,
pubmed-meshheading:16928277-Sequence Analysis, DNA,
pubmed-meshheading:16928277-Statistics, Nonparametric
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pubmed:year |
2006
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pubmed:articleTitle |
Assessment of whole genome amplification-induced bias through high-throughput, massively parallel whole genome sequencing.
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pubmed:affiliation |
454 Life Sciences, 20 Commercial Street, Branford CT 06405, USA. robert.pinard@historx.com
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, N.I.H., Extramural
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