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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2006-8-23
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pubmed:abstractText |
Non-phosphorylating glyceraldehyde- 3-phosphate dehydrogenase (NP-GAPDH) is a conserved cytosolic protein found in higher plants. In photosynthetic cells, the enzyme is involved in a shuttle transfer mechanism to export NADPH from the chloroplast to the cytosol. To investigate the role of this enzyme in plant tissues, we characterized a mutant from Arabidopsis thaliana having an insertion at the NP-GAPDH gene locus. The homozygous mutant was determined to be null respect to NP-GAPDH, as it exhibited undetectable levels of both transcription of NP-GAPDH mRNA, protein expression and enzyme activity. Transcriptome analysis demonstrated that the insertion mutant plant shows altered expression of several enzymes involved in carbohydrate metabolism. Significantly, cytosolic phosphorylating (NAD-dependent) glyceraldehyde-3-phosphate dehydrogenase mRNA levels are induced in the mutant, which correlates with an increase in enzyme activity. mRNA levels and enzymatic activity of glucose-6-phosphate dehydrogenase were also elevated, correlating with an increase in NADPH concentration. Moreover, increased ROS levels were measured in the mutant plants. Down-regulation of several glycolytic and photosynthetic genes suggests that NP-GAPDH is important for the efficiency of both metabolic processes. The results presented demonstrate that NP-GAPDH has a relevant role in plant growth and development.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0167-4412
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
945-57
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16927206-Arabidopsis,
pubmed-meshheading:16927206-Carbon Dioxide,
pubmed-meshheading:16927206-Chlorophyll,
pubmed-meshheading:16927206-Cytosol,
pubmed-meshheading:16927206-Gene Expression Regulation, Developmental,
pubmed-meshheading:16927206-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:16927206-Gene Expression Regulation, Plant,
pubmed-meshheading:16927206-Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+),
pubmed-meshheading:16927206-Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating),
pubmed-meshheading:16927206-Glycolysis,
pubmed-meshheading:16927206-Microscopy, Fluorescence,
pubmed-meshheading:16927206-Mutagenesis, Insertional,
pubmed-meshheading:16927206-Mutation,
pubmed-meshheading:16927206-NADP,
pubmed-meshheading:16927206-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:16927206-Phenotype,
pubmed-meshheading:16927206-Photosynthesis,
pubmed-meshheading:16927206-Plant Leaves,
pubmed-meshheading:16927206-RNA, Messenger,
pubmed-meshheading:16927206-Reactive Oxygen Species,
pubmed-meshheading:16927206-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16927206-Superoxides
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pubmed:year |
2006
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pubmed:articleTitle |
Characterization of an Arabidopsis thaliana mutant lacking a cytosolic non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase.
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pubmed:affiliation |
IIB-INTECH, UNSAM-CONICET, CC 164 (7130), Chascomús, Argentina.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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