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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1990-5-31
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pubmed:databankReference |
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pubmed:abstractText |
The optional Escherichia coli prr locus restricts phage T4 mutants lacking polynucleotide kinase or RNA ligase. Underlying this restriction is the specific manifestation of the T4-induced anticodon nuclease, an enzyme which triggers the cleavage-ligation of the host tRNALys. We report here the molecular cloning, nucleotide sequence and mutational analysis of prr-associated DNA. The results indicate that prr encodes a latent form of anticodon nuclease consisting of a core enzyme and cognate masking agents. They suggest that the T4-encoded factors of anticodon nuclease counteract the prr-encoded masking agents, thus activating the latent enzyme. The encoding of a tRNA cleavage-ligation pathway by two separate genetic systems which cohabitate E. coli may provide a clue to the evolution of RNA splicing mechanisms mediated by proteins.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-1067605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-210287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-2444436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-2676520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-3280805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-3323819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-3519981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-353741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-4362515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-4888151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-5331240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-6086961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-6094490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-6099322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-6296815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-6297798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1691706-7042981
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1383-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1691706-Amino Acid Sequence,
pubmed-meshheading:1691706-Base Sequence,
pubmed-meshheading:1691706-Cloning, Molecular,
pubmed-meshheading:1691706-DNA Mutational Analysis,
pubmed-meshheading:1691706-Enzyme Activation,
pubmed-meshheading:1691706-Escherichia coli,
pubmed-meshheading:1691706-Gene Expression Regulation, Bacterial,
pubmed-meshheading:1691706-Molecular Sequence Data,
pubmed-meshheading:1691706-RNA, Bacterial,
pubmed-meshheading:1691706-RNA Splicing,
pubmed-meshheading:1691706-Ribonucleases,
pubmed-meshheading:1691706-T-Phages
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pubmed:year |
1990
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pubmed:articleTitle |
The optional E. coli prr locus encodes a latent form of phage T4-induced anticodon nuclease.
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pubmed:affiliation |
Department of Biochemistry, Tel Aviv University, Ramat Aviv, Israel.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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