Linked Life Data

16905157

Source:http://linkedlifedata.com/resource/pubmed/id/16905157

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2006-10-18
pubmed:abstractText
The suitability of the comet assay for quantifying DNA repair capacity at individual level was studied following the kinetics of nucleotide excision repair (NER) in human lymphocytes from four healthy donors, at various time steps after a single dose of UVC. A significant increase of DNA migration was seen as soon as 20 min after UV exposure, reaching the peak within 60-90 min. Afterwards, a rapid decline was observed, approaching the basal level at 180-240 min. The increase could be ascribed to excision activity, while the reduction to gap filling and rejoining, as demonstrated by the effects of phase-specific inhibitors, novobiocin and aphidicolin. Therefore, the comet assay should allow following the biphasic kinetics of NER. Wide inter-individual differences were observed, although repeated tests on the same donor cells revealed a large experimental variation. To quantitatively compare the individual patterns, a mathematical model was developed that adequately fitted the experimental results and estimated appropriate descriptors for each phase and for each donor. A second approach was also used to directly compare the distributions of damaged cells and to assess the differences between donors and between experiments visualizing them as reciprocal distances on a two-dimensional space computed with a principal component analysis (PCA). The results confirmed the inter-individual differences, but also the strong influence of experimental factors of the comet assay. The two approaches provided the means of accurately comparing DNA repair kinetics at individual level, taking also into account the experimental variability which poses serious doubts on the suitability of the comet assay. Nevertheless, since this methodology allows a detailed analysis of repair kinetics and it is potentially very useful for identifying individual with reduced repair capacity, further efforts have to be addressed to improve the reproducibility of the comet assay.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-5107
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
601
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
150-61
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Can individual repair kinetics of UVC-induced DNA damage in human lymphocytes be assessed through the comet assay?
pubmed:affiliation
Dipartimento di Biologia, Università di Pisa, via Derna 1, Pisa, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't