Linked Life Data

1689854

Source:http://linkedlifedata.com/resource/pubmed/id/1689854

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1990-4-9
pubmed:abstractText
Immunologic strategies for removal of malignant cells from autologous marrow grafts by "negative selection" (i.e., "purging") requiring multiple specific monoclonal antibodies for each tumor type. "Positive selection" of marrow stem cells for grafting is a possible alternative strategy, using a monoclonal antibody which selectively recognizes lymphohematopoietic stem cells. The human hematopoietic progenitor cell antigen, CD34, is an integral cell membrane glycoprotein of approximately 115 kD, which has been molecularly cloned and sequenced. Although its function has not been determined, the glycoprotein has been characterized biochemically, including preliminary epitope mapping. Collective results from several laboratories indicate that CD34 monoclonal antibodies (My10, BI-3C5, 12.8, etc.) have the appropriate specificity to warrant testing their utility in positive selection for autologous bone marrow transplantation. First, precursors for all human hematopoietic lineages assayed (including most CFU-GM, BFU-E, CFU-MEG, CFU-EO, CFU-MIX or CFU-GEMM, pre-CFU, CFUBLAST, and terminal transferase+ B [and probably T] lymphoid precursors) are CD34+. Second, only 1.5% (mean) of low density human marrow mononuclear cells express CD34; mature human blood and marrow cells are CD34-. Endothelial cells are the only fixed tissue cells which express CD34. Third, the expression of CD34 in malignancies appears to parallel normal cellular expression: of hematopoietic malignancies, some acute leukemias and chronic myelogenous leukemia blasts are CD34+, but chronic lymphois leukemias, lymphomas, myelomas and non-hematopoietic malignancies are uniformly CD34-. Fourth, it appears feasible to isolate CD34+ cells from clinical marrow harvest samples in large scale, using either columns or immunomagnetic microspheres. Fifth, recent studies in very small numbers of non-human primates and human patients suggest that isolated CD34+ cells include the true hematopoietic stem cell, since transplantation of CD34+ cells, into myeloblated recipients results in at least short-term hematopoietic engraftment. It is anticipated that transplantation of CD34+ marrow cells may have broad applicability in clinical bone marrow transplantation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0361-7742
pubmed:author
pubmed:issnType
Print
pubmed:volume
333
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
387-401; discussion 402
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1689854-Animals, pubmed-meshheading:1689854-Antibodies, Monoclonal, pubmed-meshheading:1689854-Antigens, CD, pubmed-meshheading:1689854-Antigens, CD34, pubmed-meshheading:1689854-Antigens, Differentiation, pubmed-meshheading:1689854-Antigens, Surface, pubmed-meshheading:1689854-Bone Marrow Transplantation, pubmed-meshheading:1689854-Cell Fractionation, pubmed-meshheading:1689854-Cell Separation, pubmed-meshheading:1689854-Chymopapain, pubmed-meshheading:1689854-Colony-Forming Units Assay, pubmed-meshheading:1689854-Hematopoietic Stem Cells, pubmed-meshheading:1689854-Humans, pubmed-meshheading:1689854-Light, pubmed-meshheading:1689854-Magnetics, pubmed-meshheading:1689854-Microspheres, pubmed-meshheading:1689854-Monocytes, pubmed-meshheading:1689854-Rats, pubmed-meshheading:1689854-Scattering, Radiation
pubmed:year
1990
pubmed:articleTitle
Positive stem cell selection--basic science.
pubmed:affiliation
Johns Hopkins Oncology Center, Baltimore, Maryland 21205.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't