Linked Life Data

16894353

Source:http://linkedlifedata.com/resource/pubmed/id/16894353

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2006-9-19
pubmed:abstractText
Cell cultures representing different stages of prostatic carcinoma will be a useful tool allowing a more complete understanding of the role of individual genes in tumorigenesis. We used the androgen-regulated probasin promoter linked to the neomycin phosphotransferase (Neo) gene, to generate the ARR(2)PBneo transgenic mouse model. Development was normal and all six ARR(2)PBneo transgenic founder lines expressed the Neo gene in a prostate-specific manner. Line C, which expressed high levels of neo, was crossbred to LPB-Tag 12T-7f transgenic mice (in which the SV40 large T antigen (Tag) was targeted to the prostate by the large probasin (LPB) promoter). Three bigenic males (carrying both Neo and Tag transgenes) were identified. Prostatic lesions developed in these mice in a predictable and heritable manner, indicating that Neo did not alter Tag-induced prostate tumor development and progression. Three separate NeoTag epithelial cell strains were established from three bigenic mice. G418 selection was used to obtain immortalized epithelial cells in culture. Selected cells expressed the Neo and Tag transgenes, cytokeratins 8 and 18, and were androgen responsive for growth. To determine if these NeoTag cells maintained a similar in vivo phenotype to the 12T-7f transgenic line, tissue recombinations were made with rat urogenital sinus mesenchyme (rUGM) and grafted under the renal capsule of male nude mouse hosts. In recombinants, the three NeoTag strains developed PIN lesions and/or more extensive adenocarcinoma than seen in the 12T-7f mouse. Androgen ablation demonstrated that the grafts were androgen responsive. NeoTag cells grafted without rUGM developed undifferentiated adenocarcinoma demonstrating that prostatic stroma dictates the glandular architecture seen in the well-differentiated adenocarcinoma.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0023-6837
pubmed:author
pubmed:issnType
Print
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1074-88
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16894353-Adenocarcinoma, pubmed-meshheading:16894353-Androgen-Binding Protein, pubmed-meshheading:16894353-Androgens, pubmed-meshheading:16894353-Animals, pubmed-meshheading:16894353-Cell Transformation, Neoplastic, pubmed-meshheading:16894353-Cells, Cultured, pubmed-meshheading:16894353-Disease Models, Animal, pubmed-meshheading:16894353-Epithelial Cells, pubmed-meshheading:16894353-Hybridization, Genetic, pubmed-meshheading:16894353-Kanamycin Kinase, pubmed-meshheading:16894353-Male, pubmed-meshheading:16894353-Mice, pubmed-meshheading:16894353-Mice, Transgenic, pubmed-meshheading:16894353-Phenotype, pubmed-meshheading:16894353-Promoter Regions, Genetic, pubmed-meshheading:16894353-Prostate, pubmed-meshheading:16894353-Prostatic Intraepithelial Neoplasia, pubmed-meshheading:16894353-Prostatic Neoplasms, pubmed-meshheading:16894353-Rats, pubmed-meshheading:16894353-Receptors, Androgen
pubmed:year
2006
pubmed:articleTitle
Androgen-dependent prostate epithelial cell selection by targeting ARR(2)PBneo to the LPB-Tag model of prostate cancer.
pubmed:affiliation
Department of Urologic Surgery, Vanderbilt University Medical Center, Nashville, TN 37232-2765, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural