Linked Life Data

16894189

Source:http://linkedlifedata.com/resource/pubmed/id/16894189

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pubmed-article:16894189pubmed:issuePt 9lld:pubmed
pubmed-article:16894189pubmed:dateCreated2006-8-8lld:pubmed
pubmed-article:16894189pubmed:abstractTextAlthough homologues of the open reading frame (ORF) UL4 of herpes simplex virus 1 (Human herpesvirus 1) have been found in the genomes of all hitherto-analysed alphaherpesviruses, little is known about their function. In a project to analyse systematically, in an isogenic and standardized assay system, the gene products of the alphaherpesvirus pseudorabies virus (PrV; Suid herpesvirus 1), the PrV UL4 gene product was identified using a monospecific rabbit antiserum prepared against a bacterial fusion protein. Western blot and immunofluorescence analyses revealed that the 146 codon UL4 ORF of PrV was translated into a nuclear 15 kDa protein which was detectable from 6 h after infection of rabbit kidney cells, but was not found in purified virus particles. For functional analysis, a UL4-negative virus recombinant (PrV-DeltaUL4F) was generated by mutagenesis of an infectious full-length clone of the PrV genome in E. coli. PrV-DeltaUL4F was replication-competent in rabbit kidney cells, and plaque formation was not affected by the mutation. However, maximum virus titres of PrV-DeltaUL4F were decreased about fivefold compared with wild-type PrV, and electron microscopy of infected cells demonstrated an impairment of release of mature virions. This growth defect of PrV-DeltaUL4F could be corrected completely by propagation in UL4-expressing cells. Correlating with the inconspicuous in vitro phenotype, neurovirulence of PrV-DeltaUL4F was also not affected significantly. Thus, UL4 encodes a non-structural protein of PrV that enhances virion formation but is not essential for PrV replication in vitro or in vivo.lld:pubmed
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pubmed-article:16894189pubmed:pagination2517-25lld:pubmed
pubmed-article:16894189pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:16894189pubmed:year2006lld:pubmed
pubmed-article:16894189pubmed:articleTitleThe UL4 gene of pseudorabies virus encodes a minor infected-cell protein that is dispensable for virus replication.lld:pubmed
pubmed-article:16894189pubmed:affiliationInstitute of Molecular Biology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany. walter.fuchs@fli.bund.delld:pubmed
pubmed-article:16894189pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:16894189pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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