Source:http://linkedlifedata.com/resource/pubmed/id/16894189
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
Pt 9
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pubmed:dateCreated |
2006-8-8
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pubmed:abstractText |
Although homologues of the open reading frame (ORF) UL4 of herpes simplex virus 1 (Human herpesvirus 1) have been found in the genomes of all hitherto-analysed alphaherpesviruses, little is known about their function. In a project to analyse systematically, in an isogenic and standardized assay system, the gene products of the alphaherpesvirus pseudorabies virus (PrV; Suid herpesvirus 1), the PrV UL4 gene product was identified using a monospecific rabbit antiserum prepared against a bacterial fusion protein. Western blot and immunofluorescence analyses revealed that the 146 codon UL4 ORF of PrV was translated into a nuclear 15 kDa protein which was detectable from 6 h after infection of rabbit kidney cells, but was not found in purified virus particles. For functional analysis, a UL4-negative virus recombinant (PrV-DeltaUL4F) was generated by mutagenesis of an infectious full-length clone of the PrV genome in E. coli. PrV-DeltaUL4F was replication-competent in rabbit kidney cells, and plaque formation was not affected by the mutation. However, maximum virus titres of PrV-DeltaUL4F were decreased about fivefold compared with wild-type PrV, and electron microscopy of infected cells demonstrated an impairment of release of mature virions. This growth defect of PrV-DeltaUL4F could be corrected completely by propagation in UL4-expressing cells. Correlating with the inconspicuous in vitro phenotype, neurovirulence of PrV-DeltaUL4F was also not affected significantly. Thus, UL4 encodes a non-structural protein of PrV that enhances virion formation but is not essential for PrV replication in vitro or in vivo.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1317
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2517-25
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16894189-Animals,
pubmed-meshheading:16894189-Cell Line,
pubmed-meshheading:16894189-Escherichia coli,
pubmed-meshheading:16894189-Genes, Viral,
pubmed-meshheading:16894189-Herpesvirus 1, Suid,
pubmed-meshheading:16894189-Microscopy, Electron,
pubmed-meshheading:16894189-Mutagenesis,
pubmed-meshheading:16894189-Open Reading Frames,
pubmed-meshheading:16894189-Plasmids,
pubmed-meshheading:16894189-Rabbits,
pubmed-meshheading:16894189-Transfection,
pubmed-meshheading:16894189-Viral Proteins,
pubmed-meshheading:16894189-Virulence,
pubmed-meshheading:16894189-Virus Replication
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pubmed:year |
2006
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pubmed:articleTitle |
The UL4 gene of pseudorabies virus encodes a minor infected-cell protein that is dispensable for virus replication.
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pubmed:affiliation |
Institute of Molecular Biology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany. walter.fuchs@fli.bund.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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