Linked Life Data

16890987

Source:http://linkedlifedata.com/resource/pubmed/id/16890987

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-1-8
pubmed:abstractText
Pholiota nameko polysaccharide (PNPS-1) has been isolated and purified by enzyme hydrolysis, hot water extraction, ethanol precipitation, ion-exchange chromatography and gel-filtration column chromatography. The inhibition of bovine pancreas ribonuclease (RNase A) by PNPS-1 has been studied to elucidate the mechanism responsible for the decreased activity. PNPS-1 was effective in a linear mixed-type inhibition as suggested from the Lineweaver-Burk plot, Dixon plot and their replots. The values of K(i) and alphaK(i) were estimated as 299.92 and 545.71 microM, respectively. The alphaK(i) was greater than K(i) indicating that noncompetitive inhibition was predominant over competitive inhibition.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0141-8130
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
134-8
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Kinetics of inhibition of ribonuclease A by Pholiota Nameko polysaccharide.
pubmed:affiliation
Tianjin Key Laboratory of Food Nutrition and Safety, Faculty of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, PR China.
pubmed:publicationType
Journal Article