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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3A
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pubmed:dateCreated |
2006-7-7
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pubmed:abstractText |
RT-PCR analysis of compounds of catecholamine metabolism (in particular tyrosine hydroxylase, TH) is widely used for the detection of contaminating neuroblastoma cells in hematopoietic stem cell preparations. Due to reports in the literature showing that hematopoietic cells are also able to produce catecholamines, we investigated whether TH-RT-PCR is really suitable for this purpose.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0250-7005
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2075-80
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16827147-Catecholamines,
pubmed-meshheading:16827147-Cell Line, Tumor,
pubmed-meshheading:16827147-Dopamine beta-Hydroxylase,
pubmed-meshheading:16827147-Gene Expression Profiling,
pubmed-meshheading:16827147-Hematopoietic Stem Cells,
pubmed-meshheading:16827147-Humans,
pubmed-meshheading:16827147-Leukocytes, Mononuclear,
pubmed-meshheading:16827147-Neuroblastoma,
pubmed-meshheading:16827147-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16827147-Sensitivity and Specificity,
pubmed-meshheading:16827147-Tyrosine 3-Monooxygenase
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pubmed:articleTitle |
Pitfalls in detection of contaminating neuroblastoma cells by tyrosine hydroxylase RT-PCR due to catecholamine-producing hematopoietic cells.
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pubmed:affiliation |
Department of Hematology and Oncology, Children's University Hospital, D-72076 Tübingen, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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