Linked Life Data

16817215

Source:http://linkedlifedata.com/resource/pubmed/id/16817215

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-10-24
pubmed:abstractText
The in situ localization of nitrotyrosine, a product of the nitration of tyrosine residues by peroxynitrite, in the interface membranes from Co--Cr--Mo and Ti--Al--V prostheses provided evidence of nitric oxide-induced oxidative damage in the periprosthetic environment. In the present study, we compared the effects of different wear products from hip prostheses on the nitration of proteins in macrophages in vitro. Nitration of proteins was measured by Western blot using a polyclonal antibody directed against nitrotyrosines. Results showed that Co(2+) and Cr(3+) ions induced the nitration of a 79 +/- 4 kDa protein in a time- and dose-dependent manner. Indeed, the stimulation was significant (p < 0.05) after 24 h with 10 ppm Co(2+) and reached a plateau level between 48 and 72 h. With Cr(3+), the stimulation was significant (p < 0.05) only after 48 and 72 h. The effect of both Co(2+) and Cr(3+) ions was inhibited by glutathione monoethyl-ester that provides protection against oxidative stress. However, ultrahigh-molecular-weight-polyethylene and alumina ceramic particles had no significant effect on the nitration of proteins. Finally, the results showed that nitrated proteins are mainly found in the cytoplasmic fraction of cells and are absent from the nucleus. In conclusion, our results show that Co(2+) and Cr(3+) ions induce the nitration of cytoplasmic proteins in human U937 macrophages, suggesting that metal ions from MM prostheses have the potential to modify protein function in the periprosthetic environment and in circulating cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1549-3296
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
599-605
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Cobalt and chromium ions induce nitration of proteins in human U937 macrophages in vitro.
pubmed:affiliation
Division of Orthopaedic Surgery, McGill University, Lady Davis Institute for Medical Research, The SMBD-Jewish General Hospital, 3755 Ch. Cote Ste-Catherine, Montreal, Quebec, Canada, H3T 1E2.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't