Linked Life Data

16783573

Source:http://linkedlifedata.com/resource/pubmed/id/16783573

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2006-8-30
pubmed:abstractText
Identification of biochemical and mechanical stimuli in order to modulate the function of bladder smooth muscle cells (SMC) in viable detrusor constructs. Human bladder detrusor cells were seeded on bladder acellular matrix and cultured under different conditions. Cell viability and proliferation were assessed by fluorescent microscopic analyses. Histological, immunohistochemical and flow cytometric analyses were performed to compare growth characteristics and differentiation of SMC. The combination of medium conditioned with proliferative urothelium and mechanical stretch resulted in a more densely populated membrane. In this culture system, the expression of alpha-smooth muscle actin (alpha-SMA) and desmin were clearly induced after serum elimination. SMC-phenotype can be modulated in viable detrusor constructs by applying selected combinations of urothelial-conditioned media and mechanical stimulation under stepwise reduction and elimination of serum.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0724-4983
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
429-37
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
New approaches in the modulation of bladder smooth muscle cells on viable detrusor constructs.
pubmed:affiliation
Department of Urology, Technical University of Dresden, Fetscherstrasse 74, 01307, Dresden, Germany. Gouya.Ram-Liebig@uniklinikum-dresden.de
pubmed:publicationType
Journal Article