Source:http://linkedlifedata.com/resource/pubmed/id/16778334
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2006-6-16
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| pubmed:abstractText |
Hemopoietic cells or bone marrow-derived cells contribute to tissue formation, possibly by transdifferentiation into smooth muscle cells (SMCs) or myofibroblasts. In this study our goal is to examine the effects of transforming growth factor-beta1 (TGF-beta1) on the transdifferentiation of the monocyte/macrophage lineage into SMC-like cells. Using rat peritoneal exudate macrophages, we investigated the expression of smooth muscle-specific differentiation markers, such as alpha-smooth muscle actin, embryonic smooth muscle myosin heavy chain, and calponin. The treatment of macrophages with TGF-beta1 enhanced the expression of SMC-specific markers at day 4; after 7 days in culture, a higher level of expression (approximately 3- to 5-fold) was detected on Western blots. In contrast, TGF-beta1 decreased the expression of CD11b, which is a macrophage marker. Furthermore, we examined the effect of the TGF-beta type 1 receptor inhibitor SB-431542 and a replication-defective adenovirus construct expressing Smad7 (Adeno-Smad7), which inhibits TGF-beta signaling by interfering with the activation of other Smad proteins. Both SB-431542 and Adeno-Smad7 suppressed the expression of SMC-specific markers. These results indicated that TGF-beta signaling is essential for the transdifferentiation of macrophages into SMC-like cells. Elucidating the mechanism by which macrophages transdifferentiate into SMC-like cells may reveal new therapeutic targets for preventing vascular diseases.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-(5-benzo(1,3)dioxol-5-yl-4-pyridin...,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Benzamides,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Dioxoles,
http://linkedlifedata.com/resource/pubmed/chemical/Smad7 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Tgfb1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0916-9636
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
29
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
269-76
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16778334-Adenoviridae,
pubmed-meshheading:16778334-Animals,
pubmed-meshheading:16778334-Antigens, Surface,
pubmed-meshheading:16778334-Benzamides,
pubmed-meshheading:16778334-Biological Markers,
pubmed-meshheading:16778334-Cell Differentiation,
pubmed-meshheading:16778334-Cells, Cultured,
pubmed-meshheading:16778334-Dioxoles,
pubmed-meshheading:16778334-Dose-Response Relationship, Drug,
pubmed-meshheading:16778334-Genetic Vectors,
pubmed-meshheading:16778334-Macrophages, Peritoneal,
pubmed-meshheading:16778334-Male,
pubmed-meshheading:16778334-Myocytes, Smooth Muscle,
pubmed-meshheading:16778334-Rats,
pubmed-meshheading:16778334-Rats, Sprague-Dawley,
pubmed-meshheading:16778334-Signal Transduction,
pubmed-meshheading:16778334-Smad7 Protein,
pubmed-meshheading:16778334-Transforming Growth Factor beta,
pubmed-meshheading:16778334-Transforming Growth Factor beta1
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| pubmed:year |
2006
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| pubmed:articleTitle |
Transforming growth factor-beta signaling enhances transdifferentiation of macrophages into smooth muscle-like cells.
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| pubmed:affiliation |
Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine, Kobe, Japan.
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| pubmed:publicationType |
Journal Article
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