Linked Life Data

16775399

Source:http://linkedlifedata.com/resource/pubmed/id/16775399

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-6-15
pubmed:databankReference
pubmed:abstractText
5'-Flanking region of rlpk2 gene was cloned by using PCR-based genomic DNA walking method (Fig.1), and designated P(rlpk2) (GenBank Accession Number: AY870314). Results of sequencing analysis showed that P(rlpk2) contains several putative cis-elements that respond to CTK, ABA, GA or IAA, as well as elements that respond to different environmental stresses, such as dehydration, coldness, wounding and etc. An expression vector containing the P(rlpk2)-GUS fusion gene was constructed (Fig.2) and transferred into soybean seedlings and Micro-Tom cotyledon explants by Agrobacterium-mediated method. A strong transient expression of GUS was observed in both soybean plumule and Micro-Tom cotyledon callus (Fig.3), thus confirming the promoter activity of P(rlpk2).
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1671-3877
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
315-9
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
[Cloning and preliminary analysis of promoter of soybean receptor-like protein kinase gene rlpk2].
pubmed:affiliation
Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin 300071, China.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't