Linked Life Data

16757583

Source:http://linkedlifedata.com/resource/pubmed/id/16757583

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2006-6-7
pubmed:abstractText
Identification of individuals infected with Mycobacterium tuberculosis is essential for the control of tuberculosis (TB). The specificity of the currently used tuberculin skin test (TST) is poor because of the broad antigenic cross-reactivity of purified protein derivative (PPD) with BCG vaccine strains and environmental mycobacteria. Both ESAT-6 and CFP-10, two secretory proteins that are highly specific for M. tuberculosis complex, elicit strong T-cell responses in subjects with TB. Using an enzyme-linked immunospot (ELISPOT)-IFN-gamma assay and a restricted pool of peptides derived from ESAT-6 and CFP-10, we have previously demonstrated a high degree of specificity and sensitivity of the test for the diagnosis of TB. Here, 119 contacts of individuals with contagious TB who underwent TST and the ELISPOT-IFN-gamma assay were consecutively recruited. We compared the efficacy of the two tests in detecting latent TB infection and defined a more appropriate TST cutoff point. There was little agreement between the tests (k = 0.33, P < 0.0001): 53% of the contacts with a positive TST were ELISPOT negative, and 7% with a negative TST were ELISPOT positive. Furthermore, respectively 76 and 59% of the ELISPOT-negative contacts responded in vitro to BCG and PPD, suggesting that most of them were BCG vaccinated or infected with nontuberculous mycobacteria. The number of spot-forming cells significantly correlated with TST induration (P < 0.0001). Our in-house ELISPOT assay based on a restricted pool of highly selected peptides is more accurate than TST for identifying individuals with latent TB infection and could improve chemoprophylaxis for the control of TB.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-10517722, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-10702486, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-10764337, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-10764341, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-10792007, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-10816479, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-10823800, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-10875787, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-10992526, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-11009160, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-11133379, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-11282752, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-11438135, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-11673535, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-11826002, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-12441800, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-12466511, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-12686038, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-14586040, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-14608301, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-14764463, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-15034828, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-15130907, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-15297485, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-15305488, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-15567126, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-15610806, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-15614710, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-15817755, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-15890129, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-15941804, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-15941812, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-7575072, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-7729876, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-791969, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-8110954, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-8631702, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-8871652, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-9186152, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-9526192, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-9626599, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-9846755, http://linkedlifedata.com/resource/pubmed/commentcorrection/16757583-9952370
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1944-50
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
An in-house RD1-based enzyme-linked immunospot-gamma interferon assay instead of the tuberculin skin test for diagnosis of latent Mycobacterium tuberculosis infection.
pubmed:affiliation
Villa Marelli Institute, Lombardy Regional Reference Centre for Tuberculosis, Niguarda Hospital, Milan, Italy.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Evaluation Studies