Linked Life Data

16741227

Source:http://linkedlifedata.com/resource/pubmed/id/16741227

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2006-9-1
pubmed:abstractText
Stem cells of the corneal epithelium have been found to be located exclusively at the anatomical junction between the cornea and the conjunctiva, the limbus. Ex vivo expanded limbal epithelial cells on amniotic membrane (AM) are capable of restoring the corneal surface with limbal stem cell deficiency. Recent studies indicate that intact AM preserves the limbal epithelial phenotype and that distinct epithelial morphology is noted among various culture matrix. However, the factors in response to the interaction between limbal epithelial cells and AM were not well understood. Using Annexin V-fluorescein isothiocyanate staining, we found that human limbal epithelial cells expanded on intact human AM demonstrated fewer apoptotic cells as compared with those on plastic dishes. To identify the anti-apoptotic factors, we performed cDNA microarray analysis and showed that interleukin-1 receptor antagonist (IL-1RA) was overexpressed in cultures on intact AM, which was confirmed by reverse transcription-polymerase chain reaction (RT-PCR), real-time quantitative PCR (Q-PCR) and enzyme-linked immunosorbent assay. In addition, we also noted that the phenomenon of apoptosis detected in cultures on plastic dishes could be reversed by adding recombinant IL-1RA protein into the media, whereas apoptosis of limbal epithelial cells cultivated on intact AM could be induced by exogenous neutralizing IL-1RA neutralizing antibody. These results demonstrated that intact human AM may prevent cultured human limbal epithelial cells from undergoing apoptosis. IL-1RA might be a candidate mediator to exert as an anti-apoptotic molecule during the interaction between human limbal epithelial cells and intact human AM.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1066-5099
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2130-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16741227-Adolescent, pubmed-meshheading:16741227-Adult, pubmed-meshheading:16741227-Aged, pubmed-meshheading:16741227-Amnion, pubmed-meshheading:16741227-Annexin A5, pubmed-meshheading:16741227-Antibodies, pubmed-meshheading:16741227-Apoptosis, pubmed-meshheading:16741227-Cells, Cultured, pubmed-meshheading:16741227-Culture Media, Conditioned, pubmed-meshheading:16741227-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:16741227-Epithelial Cells, pubmed-meshheading:16741227-Fluorescein-5-isothiocyanate, pubmed-meshheading:16741227-Gene Expression Profiling, pubmed-meshheading:16741227-Humans, pubmed-meshheading:16741227-Interleukin 1 Receptor Antagonist Protein, pubmed-meshheading:16741227-Interleukin-1, pubmed-meshheading:16741227-Limbus Corneae, pubmed-meshheading:16741227-Middle Aged, pubmed-meshheading:16741227-Neutralization Tests, pubmed-meshheading:16741227-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:16741227-RNA, Messenger, pubmed-meshheading:16741227-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16741227-Sialoglycoproteins, pubmed-meshheading:16741227-Up-Regulation
pubmed:year
2006
pubmed:articleTitle
Interleukin-1 receptor antagonist (IL-1RA) prevents apoptosis in ex vivo expansion of human limbal epithelial cells cultivated on human amniotic membrane.
pubmed:affiliation
Department of Ophthalmology, Chung Gung Memorial Hospital, Keelung, Taiwan.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't