Source:http://linkedlifedata.com/resource/pubmed/id/16739112
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2006-8-14
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| pubmed:abstractText |
Centrosome abnormalities are observed in human cancers and have been associated with aneuploidy, a driving force in tumour progression. However, the exact pathways that tend to cause centrosome abnormalities have not been fully elucidated in human tumours. Using a series of 68 non-small-cell lung carcinomas and an array of in vitro experiments, the relationship between centrosome abnormalities, aneuploidy, and the status of key G1 to S-phase transition cell-cycle molecules, involved in the regulation of centrosome duplication, was investigated. Centrosome amplification and structural abnormalities were common (53%), were strongly related to aneuploidy, and, surprisingly, were even seen in adjacent hyperplastic regions, suggesting the possibility that these are early lesions in lung carcinogenesis. Cyclin E and E2F1 overexpression, but not p53 mutation, was observed to correlate with centrosome abnormalities in vivo (p = 0.029 and p = 0.015, respectively). This was further strengthened by the observation that cyclin E was specifically present in the nucleus and/or cytoplasm of the cells that contained centrosome aberrations. The cytoplasmic cyclin E signal may be attributed, in part, to the presence of truncated low-molecular-weight isoforms of cyclin E. In order to isolate the effect of cyclin E on the appearance of centrosome abnormalities, a U2OS tetracycline-repressible cyclin E cell line that has a normal centrosome profile by default was used. With this system, it was confirmed in vitro that persistent cyclin E overexpression is sufficient to cause the appearance of centrosome abnormalities.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0022-3417
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| pubmed:author |
pubmed-author:ApostolopoulosMM,
pubmed-author:BartekJJ,
pubmed-author:BartkovaJJ,
pubmed-author:DrougouAA,
pubmed-author:EvangelouKK,
pubmed-author:GorgoulisV GVG,
pubmed-author:KittasCC,
pubmed-author:KouloukoussaMM,
pubmed-author:KoutsamiM KMK,
pubmed-author:PaterasI SIS,
pubmed-author:SpartinouZZ,
pubmed-author:TsantoulisP KPK
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| pubmed:copyrightInfo |
Copyright 2006 Pathological Society of Great Britain and Ireland.
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| pubmed:issnType |
Print
|
| pubmed:volume |
209
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
512-21
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16739112-Aged,
pubmed-meshheading:16739112-Aneuploidy,
pubmed-meshheading:16739112-Carcinoma, Non-Small-Cell Lung,
pubmed-meshheading:16739112-Centrosome,
pubmed-meshheading:16739112-Chi-Square Distribution,
pubmed-meshheading:16739112-Cyclin E,
pubmed-meshheading:16739112-E2F1 Transcription Factor,
pubmed-meshheading:16739112-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:16739112-Female,
pubmed-meshheading:16739112-Gene Expression,
pubmed-meshheading:16739112-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:16739112-Genes, p53,
pubmed-meshheading:16739112-Humans,
pubmed-meshheading:16739112-In Situ Hybridization,
pubmed-meshheading:16739112-Lung Neoplasms,
pubmed-meshheading:16739112-Male,
pubmed-meshheading:16739112-Middle Aged,
pubmed-meshheading:16739112-Statistics, Nonparametric
|
| pubmed:year |
2006
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| pubmed:articleTitle |
Centrosome abnormalities are frequently observed in non-small-cell lung cancer and are associated with aneuploidy and cyclin E overexpression.
|
| pubmed:affiliation |
Molecular Carcinogenesis Group, Laboratory of Histology-Embryology, Medical School, University of Athens, Greece.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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