| pubmed-article:16730243 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16730243 | lifeskim:mentions | umls-concept:C0034493 | lld:lifeskim |
| pubmed-article:16730243 | lifeskim:mentions | umls-concept:C0032105 | lld:lifeskim |
| pubmed-article:16730243 | lifeskim:mentions | umls-concept:C0302908 | lld:lifeskim |
| pubmed-article:16730243 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:16730243 | lifeskim:mentions | umls-concept:C0037813 | lld:lifeskim |
| pubmed-article:16730243 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:16730243 | lifeskim:mentions | umls-concept:C0069725 | lld:lifeskim |
| pubmed-article:16730243 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:16730243 | pubmed:dateCreated | 2006-8-9 | lld:pubmed |
| pubmed-article:16730243 | pubmed:abstractText | An analytical method was developed for the determination of enantiomers of dencichine in plasma. Sample extraction from plasma was achieved by a solid-phase extraction (SPE) procedure using a C(18) cartridge, with carbocisteine as the internal standard. Plasma was deproteinized using inorganic acid and derivatizated before the SPE. Chiral separation of dencichine enantiomers was achieved by pre-column derivatization using o-phthaldialdehyde (OPA) and the chiral thiol N-isobutanoyl-L-cysteine (NIBC) to form diastereoisomeric isoindole derivatives that were separable by ODS column using a gradient solvent programme. The column eluent was monitored using mass spectrometry (MS). The conditions of MS detection were optimized, and selected ion monitoring was used to selectively detect D-dencichine and its arrangement isomer. High sensitivity and selectivity were obtained using this method. The limit of detection was determined to be 10 ng/ml for D-dencichine and 8 ng/ml for L-dencichine in plasma. The linearity was demonstrated over a wide range of concentrations, from 0.5 to 50 microg/ml for both enatiomers. The intra- and inter-day precision (C.V.), studied at four concentrations, was less than 7.0%. No interferences from endogenous amino acids and isomers of dencichine were found. The method was suitable for pharmacokinetic studies of dencichine enantiomers. | lld:pubmed |
| pubmed-article:16730243 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16730243 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16730243 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16730243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16730243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16730243 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16730243 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:16730243 | pubmed:issn | 1570-0232 | lld:pubmed |
| pubmed-article:16730243 | pubmed:author | pubmed-author:ZhouXinX | lld:pubmed |
| pubmed-article:16730243 | pubmed:author | pubmed-author:ZhuJingJ | lld:pubmed |
| pubmed-article:16730243 | pubmed:author | pubmed-author:ZhengHuH | lld:pubmed |
| pubmed-article:16730243 | pubmed:author | pubmed-author:LiZhangwanZ | lld:pubmed |
| pubmed-article:16730243 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16730243 | pubmed:day | 18 | lld:pubmed |
| pubmed-article:16730243 | pubmed:volume | 840 | lld:pubmed |
| pubmed-article:16730243 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16730243 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16730243 | pubmed:pagination | 124-31 | lld:pubmed |
| pubmed-article:16730243 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:16730243 | pubmed:meshHeading | pubmed-meshheading:16730243... | lld:pubmed |
| pubmed-article:16730243 | pubmed:meshHeading | pubmed-meshheading:16730243... | lld:pubmed |
| pubmed-article:16730243 | pubmed:meshHeading | pubmed-meshheading:16730243... | lld:pubmed |
| pubmed-article:16730243 | pubmed:meshHeading | pubmed-meshheading:16730243... | lld:pubmed |
| pubmed-article:16730243 | pubmed:meshHeading | pubmed-meshheading:16730243... | lld:pubmed |
| pubmed-article:16730243 | pubmed:meshHeading | pubmed-meshheading:16730243... | lld:pubmed |
| pubmed-article:16730243 | pubmed:meshHeading | pubmed-meshheading:16730243... | lld:pubmed |
| pubmed-article:16730243 | pubmed:meshHeading | pubmed-meshheading:16730243... | lld:pubmed |
| pubmed-article:16730243 | pubmed:meshHeading | pubmed-meshheading:16730243... | lld:pubmed |
| pubmed-article:16730243 | pubmed:meshHeading | pubmed-meshheading:16730243... | lld:pubmed |
| pubmed-article:16730243 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16730243 | pubmed:articleTitle | Enantioselective determination of dencichine in rabbit plasma by high-performance liquid chromatography-electrospray mass spectrometry. | lld:pubmed |
| pubmed-article:16730243 | pubmed:affiliation | School of Pharmacy, Sichuan University, Chengdu 610041, China. | lld:pubmed |
| pubmed-article:16730243 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16730243 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
