Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1991-5-6
pubmed:databankReference
pubmed:abstractText
The H circle of Leishmania species contains a 30 kb inverted duplication separated by two unique DNA segments, a and b. The corresponding H region of chromosomal DNA has only one copy of the duplicated DNA. We show here that the chromosomal segments a and b are flanked by inverted repeats (198 and 1241 bp) and we discuss how these repeats could lead to formation of H circles from chromosomal DNA. Selection of Leishmania tarentolae for methotrexate resistance indeed resulted in the de novo formation of circles with long inverted duplication, but two mutants selected for arsenite resistance contained new H region plasmids without such duplications. One of these plasmids appears due to a homologous recombination between two P-glycoprotein genes with a high degree of sequence homology. Our results show how the same DNA region in Leishmania may be amplified to give plasmids with or without long inverted duplications and apparently by different mechanisms.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-1969794, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-1970614, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2172836, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2185424, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2188107, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2234081, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2300209, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2304458, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2326258, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2344613, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2405257, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2449440, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2476435, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2569766, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2570548, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2647287, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2661014, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2662712, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2710170, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2768255, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2870814, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2898098, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2909386, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-2922290, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-3022301, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-3024103, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-3038532, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-3182826, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-3182827, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-3185551, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-3244352, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-3283115, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-3290843, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-3366118, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-3366764, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-3388020, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-3654626, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-3821723, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-4291934, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-6329026, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-6467372, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-6546423, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-6572966, http://linkedlifedata.com/resource/pubmed/commentcorrection/1672636-6855772
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1009-16
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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