Linked Life Data

1670948

Source:http://linkedlifedata.com/resource/pubmed/id/1670948

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-2-19
pubmed:abstractText
Cultured murine CD4+ T cells have been shown to differentiate into IL-2 or IL-4-producing subsets. The factors responsible for the development of CD4+ T cells which produce IL-2 but not IL-4 and cells capable of producing IL-4 but not IL-2 are unknown. Here we describe a system that allows the controlled induction of IL-2- or IL-4-producing T cells after one single round of activation. Freshly isolated CD8-depleted T cells were activated with various polyclonal T cell activators for 48 h, washed, and then expanded under different conditions. IL-2 and IL-4 production were induced by restimulation of T cells and were measured with CTLL cells that respond to both cytokines and mAb to IL-2 and IL-4. T cells produced mainly IL-2 and small amounts of IL-4 when restimulated after expansion culture for 12 days with rIL-2 alone. However, after expansion for 12 days in the presence of rIL-2 plus Con A, we observed a 30- to 100-fold up-regulation of IL-4 activity and a 100-fold down-regulation of IL-2 when assessed by responses of CTLL cells incubated with the supernatant of restimulated T cells and by responses of CTLL cells cocultured with restimulated cells. An increase of IL-4 and decrease of IL-2 was also observed when the results were based on the cell numbers at the beginning of the expansion culture. The induction of IL-4 and the down-regulation of IL-2 1) were not reproduced with alpha-methyl-mannoside-treated supernatant of Con A-stimulated spleen cells, 2) were not dependent on the presence of large numbers of APC, 3) did not result from differential consumption of lymphokines after restimulation, 4) were not due to a difference in the time course of IL-2 or IL-4 release in either T cell population, and 5) were obtained regardless of the agents used to activate or to restimulate the T cells. Because Con A remained detectable on the T cell surface and because expansion of activated T cells with IL-2 plus Con A for several days was necessary, our results indicate that mainly IL-4-producing CD4+ T cells can be induced by prolonged engagement of T cell surface molecules.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
146
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
577-84
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1670948-Animals, pubmed-meshheading:1670948-Antigen-Presenting Cells, pubmed-meshheading:1670948-CD4-Positive T-Lymphocytes, pubmed-meshheading:1670948-Concanavalin A, pubmed-meshheading:1670948-Down-Regulation, pubmed-meshheading:1670948-Female, pubmed-meshheading:1670948-Interleukin-2, pubmed-meshheading:1670948-Interleukin-4, pubmed-meshheading:1670948-Ionomycin, pubmed-meshheading:1670948-Kinetics, pubmed-meshheading:1670948-Lectins, pubmed-meshheading:1670948-Lymphocyte Activation, pubmed-meshheading:1670948-Male, pubmed-meshheading:1670948-Mice, pubmed-meshheading:1670948-Mice, Inbred BALB C, pubmed-meshheading:1670948-Mice, Inbred C3H, pubmed-meshheading:1670948-Mice, Inbred CBA, pubmed-meshheading:1670948-Phytohemagglutinins, pubmed-meshheading:1670948-Recombinant Proteins, pubmed-meshheading:1670948-Up-Regulation
pubmed:year
1991
pubmed:articleTitle
Lectin-mediated induction of IL-4-producing CD4+ T cells.
pubmed:affiliation
Department of Dermatology, Hôpital Cantonal Universitaire, Geneva, Switzerland.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, Non-U.S. Gov't